Initiating the inflammatory phase of incisional healing prior to tissue injury.
Initiating the inflammatory phase of incisional healing prior to tissue injury.
复制标题
在组织损伤之前启动切口愈合的炎症阶段。
DOI：
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发表时间：
2000
影响因子：
2.2
通讯作者：
Robson Mc
中科院分区：
文献类型：
--
作者：
Paul D. Smith;Paul D. Smith;M.Ann Kuhn;M.Ann Kuhn;Michael G. Franz;Michael G. Franz;T. Wachtel;T. Wachtel;Terry E. Wright;Terry E. Wright;Robson Mc;Robson Mc
背景
切口愈合所需的时间占术后疼痛和恢复的大部分。伤口愈合不良会进一步恶化这个过程。如果能开发出一种加速急性切口愈合的方法，患者将受益于减少伤口衰竭和更早恢复到发病前状态。
材料和方法
在大鼠皮肤模型中，使用切口前单次给药或每日4次给药进行切口前细胞因子或溶媒浸润。计划的切口部位用促炎细胞因子粒细胞-巨噬细胞集落刺激因子（GM-CSF）或血小板衍生生长因子BB（PDGF-BB）预处理，以在创伤前激活愈合的炎症阶段。在切口闭合时，一半的切口用转化生长因子β（2）（TGF-β（2））处理。对切口部位进行活检，用苏木精和伊红染色，并对炎性细胞和成纤维细胞群进行免疫组织化学染色，并测量断裂强度。
结果
用GM-CSF或PDGF-BB引发皮肤模拟伤口愈合的早期炎症阶段。巨噬细胞染色（EB 1）和成纤维细胞染色（波形蛋白）在切开前显著增加。炎症预处理以及在切口闭合时与TGF-β（2）结合的预处理协同改善了断裂强度。
结论
该研究表明，由用炎性细胞因子引发组织，然后在切口闭合时应用增殖细胞因子组成的序贯疗法几乎使急性伤口的断裂强度加倍。通过使用组织生长因子操纵伤口愈合的炎症和早期增殖阶段，有可能加速急性伤口修复并将伤口愈合轨迹向左转移。
BACKGROUND
The time required for incisional healing accounts for the majority of postoperative pain and convalescence. Impaired healing prolongs the process further. If a method for accelerating acute incisional wound healing could be developed, patients would benefit from decreased wound failure and an earlier return to their premorbid condition.
MATERIALS AND METHODS
In a rat dermal model, cytokine or vehicle infiltration prior to incision was performed using a single dose or four daily doses preincision. Planned incision sites were primed with the proinflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) or platelet-derived growth factor BB (PDGF-BB) in an effort to activate the inflammatory phase of healing prior to wounding. At the time of incision closure, one half of the incisions were treated with transforming growth factor beta(2) (TGF-beta(2)). Incisional sites were biopsied and stained with hematoxylin and eosin and immunohistochemistry for inflammatory cells and fibroblast populations and breaking strength was measured.
RESULTS
Priming skin with GM-CSF or PDGF-BB mimicked the early inflammatory phase of wound healing. Macrophage staining (EB1) and fibroblast staining (vimentin) were significantly increased prior to incision. Inflammatory priming as well as priming coupled with TGF-beta(2) at the time of the incision closure synergistically improved breaking strength.
CONCLUSION
This study demonstrates that sequential therapy consisting of priming of tissue with an inflammatory cytokine followed by application of a proliferative cytokine at the time of incision closure nearly doubles the breaking strength of an acute wound. By manipulating the inflammatory and early proliferative phases of wound healing with tissue growth factors, it may be possible to accelerate acute wound repair and shift the wound healing trajectory to the left.
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DOI：
10.1073/pnas.86.7.2229
发表时间：
1989-04-01
影响因子：
11.1
作者：
PIERCE, GF;MUSTOE, TA;DEUEL, TF
通讯作者：
DEUEL, TF
影响因子：
3.1
作者：
Hunt, TK;Hopt, HW
通讯作者：
Hopt, HW
DOI：
10.1006/jsre.1997.5178
发表时间：
1997-10
期刊：
The Journal of surgical research
影响因子：
--
作者：
L. Wu;Y. Yu;R. Galiano;S. I. Roth;Thomas A. Mustoe, MD, FACS
通讯作者：
L. Wu;Y. Yu;R. Galiano;S. I. Roth;Thomas A. Mustoe, MD, FACS
DOI：
10.1073/pnas.84.21.7696
发表时间：
1987-11-01
影响因子：
11.1
作者：
LYNCH, SE;NIXON, JC;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
影响因子：
15.9
作者：
DEUEL, TF;SENIOR, RM;HUANG, JS
通讯作者：
HUANG, JS