Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells.
Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells.
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DOI：
10.1038/srep03224
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发表时间：
2013-11-15
影响因子：
4.6
通讯作者：
Ueno, Hiroo
中科院分区：
文献类型：
--
作者：
Hisha, Hiroko;Tanaka, Toshihiro;Kanno, Shohei;Tokuyama, Yoko;Komai, Yoshihiro;Ohe, Shuichi;Yanai, Hirotsugu;Omachi, Taichi;Ueno, Hiroo
尽管建立舌上皮细胞培养体系的必要性很强，但目前还没有建立一种简便的培养方法。在这里，我们报告了一种使用三维基质和生长因子建立的新型舌上皮类器官培养系统。组织学分析表明，生成的类器官既有层状鳞状上皮细胞层，也有角质层。最近，我们通过多色谱系示踪方法显示，Bmi1阳性干细胞存在于乳头间窝上皮基底层的底部。使用我们的新的培养系统，我们发现单个Bmi1阳性的干细胞可以产生类器官，并且在建立的类器官中，在最外层产生了多个Bmi1阳性的干细胞。此外，我们观察到，在培养早期收获的有机类化合物可以在受体小鼠的舌头上植入和成熟，并且从致癌物处理的小鼠产生的有机类化合物具有异常的形态。因此，该培养体系不仅为研究舌上皮的调节机制，而且为研究舌再生和癌变提供了有价值的环境。
Despite the strong need for the establishment of a lingual epithelial cell culture system, a simple and convenient culture method has not yet been established. Here, we report the establishment of a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Histological analyses showed that the generated organoids had both a stratified squamous epithelial cell layer and a stratum corneum. Very recently, we showed via a multicolor lineage tracing method that Bmi1-positive stem cells exist at the base of the epithelial basal layer in the interpapillary pit. Using our new culture system, we found that organoids could be generated by single Bmi1-positive stem cells and that in the established organoids, multiple Bmi1-positive stem cells were generated at the outermost layer. Moreover, we observed that organoids harvested at an early point in culture could be engrafted and maturate in the tongue of recipient mice and that the organoids generated from carcinogen-treated mice had an abnormal morphology. Thus, this culture system presents valuable settings for studying not only the regulatory mechanisms of lingual epithelium but also lingual regeneration and carcinogenesis.
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DOI：
10.1634/stemcells.2008-0611
发表时间：
2009-02
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Okubo T;Clark C;Hogan BL
通讯作者：
Hogan BL
影响因子：
64.8
作者：
Rinkevich, Yuval;Lindau, Paul;Ueno, Hiroo;Longaker, Michael T.;Weissman, Irving L.
通讯作者：
Weissman, Irving L.
影响因子：
14.8
作者：
Dongari-Bagtzoglou, Anna;Kashleva, Helena
通讯作者：
Kashleva, Helena
影响因子：
11.5
作者：
Tang, XH;Knudsen, B;Gudas, LJ
通讯作者：
Gudas, LJ
影响因子：
64.8
作者：
Sato, Toshiro;van Es, Johan H.;Snippert, Hugo J.;Stange, Daniel E.;Vries, Robert G.;van den Born, Maaike;Barker, Nick;Shroyer, Noah F.;van de Wetering, Marc;Clevers, Hans
通讯作者：
Clevers, Hans