Cell lineage mapping of taste bud cells and keratinocytes in the mouse tongue and soft palate.
Cell lineage mapping of taste bud cells and keratinocytes in the mouse tongue and soft palate.
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DOI：
10.1634/stemcells.2008-0611
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Hogan BL
中科院分区：
文献类型：
--
作者：
Okubo T;Clark C;Hogan BL
小鼠舌和软腭的上皮至少由三种不同的上皮细胞组成：基底层细胞、组成丝状和真菌状乳头的角化细胞，以及在真菌状和环状乳头和软腭中以紧密的味蕾形式存在的味觉感受器细胞。所有这三种类型的细胞都是从胚胎舌和腭部的简单上皮发育而来，并在成年后不断被细胞更替所取代。以前在成年小鼠身上使用脉冲追逐氚胸苷标记的研究提供了角质形成细胞(5-7天)和味蕾(10天)高细胞周转率的证据。然而，在这些味觉组织中，产生成熟味蕾细胞和周围角质形成细胞的长期干细胞或祖细胞的定位和表型知之甚少。在这里，我们利用他莫昔芬可诱导的K14-Creer转基因和rosa26LacZ报告等位基因来追踪出生后早期和成年小鼠舌头和软腭的成熟角质形成细胞和味蕾细胞。我们的结果支持这一假设，即味蕾的毛孔角质形成细胞和受体细胞都来自位于味蕾外的共同的K14+K5+Trp63+Sox2+双潜祖细胞群体。这一结果也与丝状和真菌状乳头的角质形成细胞来源于位于这些结构底部的基底祖细胞的模型相一致。
The epithelium of the mouse tongue and soft palate consists of at least three distinct epithelial cell populations: basal cells, keratinized cells organized into filiform and fungiform papillae, and taste receptor cells present in tight clusters known as taste buds in the fungiform and circumvallate papillae and soft palate. All three cell types develop from the simple epithelium of the embryonic tongue and palate, and are continually replaced in the adult by cell turnover. Previous studies using pulse-chase tritiated thymidine labeling in the adult mouse provided evidence for a high rate of cell turnover in the keratinocytes (5–7 days) and taste buds (10 days). However, little is known about the localization and phenotype of the long-term stem or progenitor cells that give rise to the mature taste bud cells and surrounding keratinocytes in these gustatory tissues. Here, we make use of a tamoxifen-inducible K14-CreER transgene and the ROSA26 LacZ reporter allele to lineage trace the mature keratinocytes and taste bud cells of the early postnatal and adult mouse tongue and soft palate. Our results support the hypothesis that both the pore keratinocytes and receptor cells of the taste bud are derived from a common K14+K5+Trp63+Sox2+ population of bipotential progenitor cells located outside the taste bud. The results are also compatible with models in which the keratinocytes of the filiform and fungiform papillae are derived from basal progenitor cells localized at the base of these structures.
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