Effect of electroacupuncture on c-IAP1 mRNA and c-IAP2 mRNA in synovial tissues of rats with adjuvant arthritis
Effect of electroacupuncture on c-IAP1 mRNA and c-IAP2 mRNA in synovial tissues of rats with adjuvant arthritis
复制标题
电针对佐剂性关节炎大鼠滑膜组织c-IAP1 mRNA和c-IAP2 mRNA的影响
DOI：
10.1007/s11726-019-1088-9
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发表时间：
2019-02
影响因子：
0.5
通讯作者：
Yu Zhao-an
中科院分区：
文献类型：
--
作者：
Ai Kun;Li Yan-ling;Qi Fang;Liu Li;Chang Xiao-rong;Yu Zhao-an
目的观察电针足三里、关元、阿是穴对佐剂性关节炎大鼠的治疗作用，探讨电针治疗类风湿关节炎的作用机制。方法将60只雄性大鼠随机分为正常组、模型组、甲氨蝶呤组和电针组，每组15只。正常组和模型组常规饲养，不治疗；甲氨蝶呤组大鼠给予甲氨蝶呤0.35 mg/(Kgbw)，每周2次，连续3周；电针组大鼠针刺足三里、关元、阿是穴，双侧足三里、阿是穴与电针仪相连，1次/d，连续3周。观察各组大鼠一般情况、足趾肿胀度、关节炎指数(AI)评分、踝关节病理形态、关节滑膜组织细胞细胞凋亡抑制蛋白(c-IAP)1、c-IAP2的mRNA表达。结果模型组大鼠足趾肿胀度、AI评分及c-IAP1、c-IAP2的mRNA表达均显著高于正常组(均P<0.05)。与模型组比较，甲氨蝶呤组和电针组大鼠足趾肿胀度、AI评分及c-IAP1、c-IAP2基因表达均有改善(P<；0.01或P<；0.05)，电针组大鼠滑膜组织c-IAP1、c-IAP2基因表达明显高于甲氨蝶呤组(P<；0.01)。结论电针可减轻佐剂性关节炎大鼠关节肿胀。其作用机制可能与抑制c-IAP1、c-IAP2基因的表达，从而诱导滑膜细胞凋亡有关。
ObjectiveTo observe the therapeutic effect of electroacupuncture (EA) at Zusanli (ST 36), Guanyuan (CV 4) and Ashi points on adjuvant arthritis rats, and explore the mechanism of EA treatment of rheumatoid arthritis (RA).MethodsSixty male rats were randomly divided into a normal group, a model group, a methotrexate group and an EA group, with 15 rats in each group. Rats in the normal group and the model group were routinely raised and did not receive treatment; rats in the methotrexate group received methotrexate at a dose of 0.35 mg/(kgbw), twice a week for 3 weeks; rats in the EA group received acupuncture at Zusanli (ST 36), Guanyuan (CV 4) and Ashi points, and the bilateral Zusanli (ST 36) and Ashi points were connected to EA apparatus, once a day for 3 weeks. The general status, the swelling degree of the toe, the arthritis index (AI) score, the pathological morphology of the ankle joint, and the mRNA expressions of cellular inhibitor of apoptosis protein (c-IAP) 1 and c-IAP2 in joint synovial tissue cells of the rats in each group were observed.ResultsThe swelling degree of the toe, AI score and mRNA expressions of c-IAP1 and c-IAP2 in the model group were significantly higher than those in the normal group (all P<0.05). Compared with the model group, the swelling degree of the toe, AI score and mRNA expressions of c-IAP1 and c-IAP2 in the methotrexate group and the EA group improved (P<0.01 or P<0.05); the expressions of c-IAP1 mRNA and c-IAP2 mRNA in rat synovial tissues in the EA group were significantly higher than those in the methotrexate group (P<0.01).ConclusionEA alleviates joint swelling in rats with adjuvant arthritis. The mechanism may be related to suppressing mRNA expressions of c-IAP1 and c-IAP2, thus to induce apoptosis of synoviocytes.
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DOI：
10.1016/s0278-5846(03)00017-4
发表时间：
2003-04
影响因子：
5.6
作者：
A. LeBlanc
通讯作者：
A. LeBlanc
影响因子：
3.8
作者：
Shi DL;Shi GR;Xie J;Du XZ;Yang H
通讯作者：
Yang H
影响因子：
0.5
作者：
Hui Zhang;Xiao-Peng Ma;Huan-gan Wu;Shengbing Wu;San-shan Su;Yang-chun Hu;Zhen-feng Li;Li Zhang;Ming-yue Xie
通讯作者：
Hui Zhang;Xiao-Peng Ma;Huan-gan Wu;Shengbing Wu;San-shan Su;Yang-chun Hu;Zhen-feng Li;Li Zhang;Ming-yue Xie
影响因子：
13.3
作者：
Arvikar, Sheila L.;Crowley, Jameson T.;Sulka, Katherine B.;Steere, Allen C.
通讯作者：
Steere, Allen C.
影响因子：
--
作者：
Stanford, Stephanie M.;Maestre, Michael F.;Campbell, Amanda M.;Bartok, Beatrix;Kiosses, William B.;Boyle, David L.;Arnett, Heather A.;Mustelin, Tomas;Firestein, Gary S.;Bottini, Nunzio
通讯作者：
Bottini, Nunzio