Low concentration of condensed tannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7 cells.
Low concentration of condensed tannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7 cells.
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儿茶中的低浓度缩合单宁显着抑制脂肪酸合成酶和 MCF-7 细胞的生长。
DOI：
10.1016/j.bbrc.2008.04.062
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发表时间：
2008-07
影响因子：
3.1
通讯作者：
中科院分区：
文献类型：
--
作者：
单宁广泛存在于植物中，但因为它们能沉淀蛋白质，科学家在寻找生物活性成分时经常忽略它们。儿茶是一种传统的收敛剂，含有丰富的单宁。在这项研究中，我们发现儿茶中的缩合单宁能有效地抑制动物脂肪酸合成酶(FAS)。其中，三聚体缩合单宁的抑制作用最强，IC50为0.47μg/ml，且具有较强的时间依赖性。其对Fas的抑制动力学和作用部位与已知的Fas抑制剂有明显不同。此外，缩合单宁还能抑制MCF-7乳腺癌细胞的生长，其作用与其抑制Fas活性有关。低浓度的缩合单宁对Fas活性和MCF-7细胞生长均有抑制作用，而Fas未被沉淀。这些结果表明，单宁是一种有价值的生物活性物质来源。
Tannins exist widely in plants, but because they precipitate proteins, scientists frequently ignore them in search of bioactive components. Catechu, a traditional astringent, is rich in tannins. In this study, we found that condensed tannins from catechu potently inhibited animal fatty acid synthase (FAS). Among them, trimeric condensed tannin showed the most potent inhibition with IC50of 0.47μg/ml and it also exhibited strong time-dependent inhibition. Its inhibitory kinetics and reacting sites on FAS were obviously different from the known inhibitors of FAS. Furthermore, condensed tannins were found to suppress the growth of MCF-7 breast cancer cells, and the effect was related to their activity of FAS inhibition. The inhibition of both FAS activity and MCF-7 growth was exhibited by low concentrations of condensed tannins without FAS being precipitated. These results suggest tannins would be a valuable resource of bioactive substances.
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影响因子：
1.6
作者：
N. Vivas;M. Nonier;I. Pianet;N. V. D. Gaulejac;E. Fouquet
通讯作者：
N. Vivas;M. Nonier;I. Pianet;N. V. D. Gaulejac;E. Fouquet
影响因子：
6.1
作者：
L. Butler
通讯作者：
L. Butler
DOI：
10.1007/springerreference_173788
发表时间：
2001-02
期刊：
Nihon rinsho. Japanese journal of clinical medicine
影响因子：
--
作者：
A. Kawaguchi
通讯作者：
A. Kawaguchi
影响因子：
6.1
作者：
HAGERMAN, AE;BUTLER, LG
通讯作者：
BUTLER, LG
影响因子：
6.4
作者：
Brusselmans, K;De Schrijver, E;Swinnen, JV
通讯作者：
Swinnen, JV