A continuous-flow, microfluidic fraction collection device.
A continuous-flow, microfluidic fraction collection device.
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DOI：
10.1016/j.chroma.2010.05.023
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发表时间：
2010-07-09
期刊：
影响因子：
--
通讯作者：
Roper MG
中科院分区：
文献类型：
--
作者：
Baker CA;Roper MG
提出了一种执行电泳分离和级分收集的微流体装置。来自 3.5 cm 分离通道的流出物通过两个鞘流通道集中到七个收集通道之一。通过将收集通道保持在地电位并改变两个鞘流通道处的电压比，分离流出物被引导至特定的收集通道，或者可以在规定的时间段内扫过所有通道。由于施加到两个鞘流通道的电压之和恒定，因此无论使用哪种收集通道，在分离过程中电场都保持在 275 V/cm。分离通道中的恒定电势允许在收集早期迁移峰的同时不间断地分离晚期迁移峰。为了最大限度地减少馏分之间残留的可能性，使用三级因子模型优化了装置的几何形状。最佳条件是鞘流通道和分离通道之间的夹角为22.5°，分离出口和级分通道之间的通道长度为350 µm。使用这些优化的尺寸，通过荧光标记的氨基酸混合物的分离和级分收集来评估装置性能。在微流体平台上收集馏分的能力在这些设备的自动化或连续操作或收集珍贵样品期间特别有用。
A microfluidic device is presented that performs electrophoretic separation coupled with fraction collection. Effluent from the 3.5 cm separation channel was focused via two sheath flow channels into one of seven collection channels. By holding the collection channels at ground potential and varying the voltage ratio at the two sheath flow channels, the separation effluent was directed to either specific collection channels, or could be swept past all channels in a defined time period. As the sum of the voltages applied to the two sheath flow channels was constant, the electric field remained at 275 V/cm during the separation regardless of the collection channel used. The constant potential in the separation channel allowed uninterrupted separation for late-migrating peaks while early-migrating peaks were being collected. To minimize the potential for carryover between fractions, the device geometry was optimized using a three-level factorial model. The optimum conditions were a 22.5° angle between the sheath flow channels and the separation channel, and a 350 µm length of channel between the separation outlet and the fraction channels. Using these optimized dimensions, the device performance was evaluated by separation and fraction collection of a fluorescently-labeled amino acid mixture. The ability to fraction collect on a microfluidic platform will be especially useful during automated or continuous operation of these devices or to collect precious samples.
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