Integrated microfluidic device for automated single cell analysis using electrophoretic separation and electrospray ionization mass spectrometry.
Integrated microfluidic device for automated single cell analysis using electrophoretic separation and electrospray ionization mass spectrometry.
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DOI：
10.1021/ac902218y
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发表时间：
2010-02-01
影响因子：
7.4
通讯作者：
Ramsey, J. Michael
中科院分区：
文献类型：
--
作者：
Mellors, J. Scott;Jorabchi, Kaveh;Smith, Lloyd M.;Ramsey, J. Michael
开发了一种微加工流体装置，用于使用毛细管电泳（CE）和电喷雾电离质谱（ESI-MS）对单个细胞进行自动化实时分析。微流体结构结合了用于在游离溶液电泳通道内快速裂解单细胞的装置，其中细胞成分被分离，以及用于电离分离的成分的集成电喷雾发射器。使用质谱法表征洗脱液。使用人红细胞作为本研究的模型系统。在这种单片集成装置中，使用快速缓冲液交换和电场强度增加的组合在通道交叉处发生细胞裂解。在电泳分离通道的端部结合有电渗泵，以将洗脱液引导至集成的电喷雾发射器。当细胞连续流过该装置时，检测来自单个红细胞的游离血红素基团和血红蛋白的α和β亚基。平均分析通量约为每分钟12个细胞，证明了该方法用于高通量单细胞分析的潜力。
A microfabricated fluidic device was developed for the automated real-time analysis of individual cells using capillary electrophoresis (CE) and electrospray ionization-mass spectrometry (ESI-MS). The microfluidic structure incorporates a means for rapid lysis of single cells within a free solution electrophoresis channel where cellular constituents were separated and an integrated electrospray emitter for ionization of separated components. The eluent was characterized using mass spectrometry. Human erythrocytes were used as a model system for this study. In this monolithically integrated device, cell lysis occurs at a channel intersection using a combination of rapid buffer exchange and an increase in electric field strength. An electroosmotic pump is incorporated at the end of the electrophoretic separation channel to direct eluent to the integrated electrospray emitter. The dissociated heme group and the α and β subunits of hemoglobin from individual erythrocytes were detected as cells continuously flowed through the device. The average analysis throughput was approximately 12 cells per minute demonstrating the potential of this method for high-throughput single cell analysis.
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影响因子：
7.4
作者：
Mellors, J. S.;Gorbounov, V.;Ramsey, R. S.;Ramsey, J. M.
通讯作者：
Ramsey, J. M.
影响因子：
7.4
作者：
Chen, SJ;Lillard, SJ
通讯作者：
Lillard, SJ
DOI：
10.1016/0006-291x(63)90132-3
发表时间：
1963-01-01
影响因子：
3.1
作者：
HARSHMAN, S;NAJJAR, VA
通讯作者：
NAJJAR, VA
DOI：
10.1016/s0378-4347(99)00539-3
发表时间：
2000-04-28
影响因子：
3
作者：
Krylov, SN;Arriaga, E;Dovichi, NJ
通讯作者：
Dovichi, NJ
影响因子：
2.8
作者：
Akbar, SA;Brown, PR
通讯作者：
Brown, PR