Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis
Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis
复制标题
Interleukin-1 超家族基因在正常或受损的人类精子发生中的表达
作者：
N. Rozwadowska;D. Fiszer;P. Jędrzejczak;W. Kosicki;M. Kurpisz
白细胞介素-1（IL-1）是一种多效性细胞因子，可能在哺乳动物睾丸的特异性免疫环境和调节细胞分化中发挥作用。我们已经确定了IL-1基因家族的转录活性（使用实时聚合酶链反应（PCR））在两个主要的功能睾丸隔室（间质和小管内的），并在组织匀浆中获得的患者生育障碍（生精障碍和睾丸肿瘤）。我们观察到显著表达的基因编码的IL-1受体拮抗剂（IL-1 RA）的配子发生细胞（正常性腺）的纯化部分。Caspase-1（ICE - IL-1β-转换酶）在间质区室以及睾丸肿瘤中高度表达（mRNA水平）（免疫增强？）。此外，我们还发现IL-1 RA基因活性沿着生精功能的改变而降低，并与IL-1α呈负相关（从正常性腺到生精停滞再到唯支持细胞综合征）。因此，IL-1α/IL-1 RA比值有可能作为精子发生的诊断性分子探针。IL-1-IL-18细胞因子及其受体，特别是免疫赦免性腺中受体拮抗剂的mRNA的精确水平，可能是负责维持睾丸稳态的主要因素之一，从而使成熟精子的产生成为可能。
Interleukin-1 (IL-1) is a pleiotropic cytokine that may play a role in contributing to the specific immune environment of mammalian testis and in regulating cell differentiation. We have determined the transcription activity of the IL-1 gene family (using real-time polymerase chain reaction (PCR)) in two main functional testicular compartments (interstitial and intratubular ones), and in tissue homogenates obtained from patients with fertility disorders (spermatogenic arrest and testicular tumors). We observed the prominent expression of gene coding for IL-1 receptor antagonist (IL-1RA) in a purified fraction of gametogenic cells (normal gonad). Caspase-1 (ICE – IL-1β-converting enzyme) was highly expressed (on mRNA level) in interstitial compartments as well in testicular tumors (immune enhancement?). In addition we found, that the activity of IL-1RA gene decreased along spermatogenic alteration in an inversely related manner with IL-1α (from normal gonad through spermatogenic arrest to Sertoli cell only syndrome). Therefore, the quotient value of IL-1α/IL-1RA could potentially serve as the diagnostic molecular probe for spermatogenesis assessment. The precise level of mRNA for IL-1–IL-18 cytokines and their receptors, and specifically of the receptor antagonist in immune privileged gonad, could be one of the main factors responsible for maintaining testicular homeostasis, thus enabling generation of the mature spermatozoa.
影响因子：
3.5
作者：
K. Svechnikov;DM Stocco;O. Soder
通讯作者：
K. Svechnikov;DM Stocco;O. Soder