H(2)S biotreatment with sulfide-oxidizing heterotrophic bacteria.
H(2)S biotreatment with sulfide-oxidizing heterotrophic bacteria.
复制标题
硫化物氧化异养细菌生物处理 H2S
DOI：
10.1007/s10532-018-9849-6
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发表时间：
2018-12
期刊：
影响因子：
3.6
通讯作者：
Xun L
中科院分区：
文献类型：
--
作者：
Hou N;Xia Y;Wang X;Liu H;Liu H;Xun L
许多工业活动都会产生硫化氢，高浓度的硫化氢是有毒的，即使浓度很低也有臭味。化化石养型硫氧化菌常用于其修复。最近，我们报道了许多异养细菌可以利用硫化物：醌氧化还原酶和过硫双加氧酶将H2S氧化为硫代硫酸盐和亚硫酸盐。这些细菌也有可能用于H2S生物处理。在这里，我们报道了如何将具有这些酶的各种异养细菌与有机化合物一起培养，并且细胞能够迅速将H2S氧化为零价硫和硫代硫酸盐，而不会引起明显的酸化。有些还将产生的硫代硫酸盐转化为四硫代酸盐。在pH 7.4条件下，部分被测细菌的硫化物氧化速率为细胞干重的8 ~ 50µmol min - 1g−1，足以进行H2S生物处理。固定化细菌去除H2S的效率与悬浮液中的细菌一样高，并且在固定化过程中包裹Fe3O4纳米颗粒导致硫化物去除效率提高，部分原因是Fe3O4对H2S进行了化学氧化。因此，异养细菌可用于好氧条件下的H2S生物处理。
Many industrial activities produce H2S, which is toxic at high levels and odorous at even very low levels. Chemolithotrophic sulfur-oxidizing bacteria are often used in its remediation. Recently, we have reported that many heterotrophic bacteria can use sulfide:quinone oxidoreductase and persulfide dioxygenase to oxidize H2S to thiosulfate and sulfite. These bacteria may also potentially be used in H2S biotreatment. Here we report how various heterotrophic bacteria with these enzymes were cultured with organic compounds and the cells were able to rapidly oxidize H2S to zero-valence sulfur and thiosulfate, causing no apparent acidification. Some also converted the produced thiosulfate to tetrathionate. The rates of sulfide oxidation by some of the tested bacteria in suspension, ranging from 8 to 50 µmol min−1g−1of cell dry weight at pH 7.4, sufficient for H2S biotreatment. The immobilized bacteria removed H2S as efficiently as the bacteria in suspension, and the inclusion of Fe3O4nanoparticles during immobilization resulted in increased efficiency for sulfide removal, in part due to chemical oxidation H2S by Fe3O4. Thus, heterotrophic bacteria may be used for H2S biotreatment under aerobic conditions.
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