Chromosome condensation may enhance X-ray-related cell lethality in a temperature-sensitive mutant (tsBN2) of baby hamster kidney cells (BHK21).
Chromosome condensation may enhance X-ray-related cell lethality in a temperature-sensitive mutant (tsBN2) of baby hamster kidney cells (BHK21).
复制标题
染色体浓缩可能会增强幼仓鼠肾细胞 (BHK21) 温度敏感突变体 (tsBN2) 中与 X 射线相关的细胞致死率。
作者：
H. Sasaki;T. Nishimoto
在tsBN 2细胞系中，其在染色体浓缩的调节机制中具有温度敏感性缺陷，通过在X射线照射后在非允许温度（40摄氏度）下孵育细胞来增强X射线的致死作用。在放线菌酮的存在下，这种增强被抑制，抑制诱导过早的染色体凝聚。在40 ℃延迟孵育的情况下和在同步化细胞中获得的发现表明，X射线相关的潜在致死性损伤（其可以通过染色体浓缩来表达）在细胞周期的任何阶段的细胞中产生，但是对于除了在G2-M期晚期周围的那些细胞之外的所有细胞都是可修复的，其中染色体凝聚发生在允许温度（33.5摄氏度）。这些观察结果表明，晚期G2-M细胞对X射线的高敏感性是由染色体浓缩相关事件引起的。
In the tsBN2 cell line, which has a temperature-sensitive defect in the regulatory mechanism for chromosome condensation, the lethal effect of X rays was enhanced by incubating the cells at a nonpermissive temperature (40 degrees C) following X irradiation. This enhancement was suppressed in the presence of cycloheximide, which inhibits induction of premature chromosome condensation. The findings obtained in the case of delayed incubation at 40 degrees C and in synchronized cells indicate that X-ray-related potentially lethal damage, which can be expressed by chromosome condensation, is produced in the cells at any stage of the cell cycle, but it is repairable for all cells except those at around the late G2-M phase, where chromosome condensation occurs at a permissive temperature (33.5 degrees C). These observations suggest that the high sensitivity of late G2-M cells to X rays is caused by the events associated with chromosome condensation.
两种形式的潜在致命损伤在平台期和对数期细胞中具有相似的修复动力学。
DOI：
10.1080/09553008514550801
发表时间：
1985
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
Utsumi,H;Elkind,MM
通讯作者：
Elkind,MM