Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex.
Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex.
复制标题
酿酒酵母核孔复合体内环的近原子结构
DOI：
10.1038/s41422-022-00632-y
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发表时间：
2022-05
期刊：
影响因子：
44.1
通讯作者：
Sui, Sen-Fang
中科院分区：
文献类型：
--
作者：
Li, Zongqiang;Chen, Shuaijiabin;Zhao, Liang;Huang, Guoqiang;Pi, Xiong;Sun, Shan;Wang, Peiyi;Sui, Sen-Fang
核孔复合体(NPC)介导真核细胞中物质的双向核质转运。然而，由于其庞大的尺寸和高度动态的性质，其准确的分子排列仍然是一个谜。在这里，我们用单粒子冷冻电子显微镜在3.73 的分辨率下确定了内环的不对称单元(IR单体)的结构，并建立了由8个核孔素的192个分子组成的完整IR的原子模型。在每个IR单体中，中间层的Z形Nup188-Nup192复合体被内外两个近似平行的菱形结构夹在中间，而Nup188、Nup192和Nic96连接所有亚基，构成一个相对稳定的IR单体。相反，完整的IR是通过IR单体之间松散和不稳定的相互作用组装的。这些结构结合以前报道的IR的结构信息，揭示了IR单体之间的两种不同的相互作用模式和IR组装中广泛的柔性连接，为IR的稳定性和延展性提供了结构基础。
Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular arrangement of NPCs remains enigmatic owing to their huge size and highly dynamic nature. Here we determined the structure of the asymmetric unit of the inner ring (IR monomer) at 3.73 Å resolution by single-particle cryo-electron microscopy, and created an atomic model of the intact IR consisting of 192 molecules of 8 nucleoporins. In each IR monomer, the Z-shaped Nup188–Nup192 complex in the middle layer is sandwiched by two approximately parallel rhomboidal structures in the inner and outer layers, while Nup188, Nup192 and Nic96 link all subunits to constitute a relatively stable IR monomer. In contrast, the intact IR is assembled by loose and instable interactions between IR monomers. These structures, together with previously reported structural information of IR, reveal two distinct interaction modes between IR monomers and extensive flexible connections in IR assembly, providing a structural basis for the stability and malleability of IR.
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影响因子：
14.8
作者：
Gietz, R. Daniel;Schiestl, Robert H.
通讯作者：
Schiestl, Robert H.
影响因子：
64.8
作者：
Kim SJ;Fernandez-Martinez J;Nudelman I;Shi Y;Zhang W;Raveh B;Herricks T;Slaughter BD;Hogan JA;Upla P;Chemmama IE;Pellarin R;Echeverria I;Shivaraju M;Chaudhury AS;Wang J;Williams R;Unruh JR;Greenberg CH;Jacobs EY;Yu Z;de la Cruz MJ;Mironska R;Stokes DL;Aitchison JD;Jarrold MF;Gerton JL;Ludtke SJ;Akey CW;Chait BT;Sali A;Rout MP
通讯作者：
Rout MP
影响因子：
16.6
作者：
Eibauer, Matthias;Pellanda, Mauro;Turgay, Yagmur;Dubrovsky, Anna;Wild, Annik;Medalia, Ohad
通讯作者：
Medalia, Ohad
影响因子：
16.8
作者：
Kelley K;Knockenhauer KE;Kabachinski G;Schwartz TU
通讯作者：
Schwartz TU
影响因子：
16.2
作者：
Coyne AN;Zaepfel BL;Hayes L;Fitchman B;Salzberg Y;Luo EC;Bowen K;Trost H;Aigner S;Rigo F;Yeo GW;Harel A;Svendsen CN;Sareen D;Rothstein JD
通讯作者：
Rothstein JD