Receptor-mediated actions of growth hormone releasing factor on granulosa cell differentiation.
Receptor-mediated actions of growth hormone releasing factor on granulosa cell differentiation.
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生长激素释放因子对颗粒细胞分化的受体介导作用。
作者：
C. Moretti;A. Bagnato;N. Solan;G. Frajese;K. Catt
在治疗不孕症时，GRF与FSH联合使用可促进卵泡成熟和排卵。这种作用可以通过刺激GH分泌和胰岛素样生长因子I的产生来介导，但已知的结构相关激素血管活性肠肽（VIP）对颗粒细胞功能的作用表明，GRF也可能直接作用于卵巢以刺激卵泡发育。在未成熟雌激素处理大鼠的颗粒细胞中进行的放射性配体结合和活化研究显示，卵巢中VIP和大鼠（r）GRF的共同受体。[125 I]VIP与颗粒细胞的特异性结合是饱和的，并依赖于时间和温度。VIP相关肽抑制放射性配体结合的相对效力为：VIP大于rGRF大于肽组氨酸异亮氨酸酰胺大于[His 1，Nle 27]人GRF（1-32）NH 2大于促胰液素。在与强效GRF激动剂[125 I] [His 1，Nle 27]GRF（1-32）NH 2的结合研究中，相对效价为：rGRF（1-43）OH大于[His 1，Nle 27]人GRF（1-32）NH 2大于VIP大于肽组氨酸异亮氨酸酰胺大于胰泌素。胰高血糖素和胃抑制肽，胰高血糖素超家族的其他肽，以及不相关的肽，包括CRF和β-内啡肽，不抑制任何放射性配体与卵巢受体的结合。在培养的颗粒细胞中，rGRF和VIP刺激cAMP的形成，其受体的腺苷酸环化酶系统的耦合一致，并增强FSH诱导的cAMP的生产。这两种肽也扩增FSH诱导的孕酮生物合成，芳香化酶活性，和LH受体的形成。这些观察结果表明，rGRF是一种有效的cAMP介导的激动剂在大鼠卵巢和成熟的颗粒细胞中的共同VIP/GRF受体的行为。很可能，GRF对促性腺激素刺激的体内卵泡发育的增强作用部分是由肽对VIP/GRF受体的直接作用介导的。此外，由于GRF存在于性腺中，因此可能局部产生的肽通过旁分泌调节FSH对颗粒细胞功能的刺激作用来促进卵泡成熟。
GRF promotes follicular maturation and ovulation when administered with FSH in the treatment of infertility. Such actions could be mediated by stimulation of GH secretion and insulin-like growth factor I production, but the known actions of the structurally related hormone, vasoactive intestinal peptide (VIP), on granulosa cell function suggested that GRF may also act directly on the ovary to stimulate follicular development. Radioligand binding and activation studies, performed in granulosa cells from immature estrogen-treated rats, revealed a common receptor for VIP and rat (r) GRF in the ovary. Specific binding of [125I]VIP to granulosa cells was saturable and dependent on time and temperature. The relative potencies of VIP-related peptides for inhibition of radioligand binding were: VIP greater than rGRF greater than peptide histidine isoleucinamide greater than [His1,Nle27] human GRF(1-32)NH2 greater than secretin. In binding studies with the potent GRF agonist, [125I] [His1,Nle27]GRF(1-32)NH2, relative potencies were: rGRF(1-43)OH greater than [His1,Nle27]human GRF(1-32)NH2 greater than VIP greater than peptide histidine isoleucinamide greater than secretin. Glucagon and gastric inhibitory peptide, other peptides of the glucagon superfamily, and unrelated peptides including CRF and beta-endorphin, did not inhibit binding of either radioligand to ovarian receptors. In cultured granulosa cells, rGRF and VIP stimulated cAMP formation, consistent with coupling of their receptors to the adenylate cyclase system, and potentiated FSH-induced cAMP production. Both peptides also amplified FSH-induced progesterone biosynthesis, aromatase activity, and LH receptor formation. These observations demonstrate that rGRF is a potent cAMP-mediated agonist in the rat ovary and acts on a common VIP/GRF receptor in maturing granulosa cells. It is likely that the potentiating effect of administered GRF on gonadotropin-stimulated follicular development in vivo is in part mediated by direct actions of the peptide on the VIP/GRF receptor. Also, since GRF is present in the gonads, it is possible that the locally-produced peptide promotes follicular maturation by paracrine modulation of the stimulatory action of FSH on granulosa cell function.
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血管活性肠多肽优先结合大鼠和兔肠细胞的基底外侧膜。
DOI：
10.1172/jci110748
发表时间：
1983
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Dharmsathaphorn,K;Harms,V;Yamashiro,DJ;Hughes,RJ;Binder,HJ;Wright,EM
通讯作者：
Wright,EM
血管活性肠肽诱导培养的大鼠卵巢颗粒细胞中胆固醇侧链裂解酶复合物的合成。
DOI：
10.1073/pnas.83.19.7490
发表时间：
1986
影响因子：
11.1
作者：
Trzeciak,WH;Ahmed,CE;Simpson,ER;Ojeda,SR
通讯作者：
Ojeda,SR
影响因子：
4.8
作者：
ADASHI, EY;RESNICK, CE;VANWYK, JJ
通讯作者：
VANWYK, JJ
生长激素释放因子。
影响因子：
16.6
作者：
Ling,N;Zeytin,F;Bohlen,P;Esch,F;Brazeau,P;Wehrenberg,WB;Baird,A;Guillemin,R
通讯作者：
Guillemin,R
大鼠胎盘中具有免疫反应性和生物活性的生长激素释放因子。
影响因子：
4.8
作者：
Baird,A;Wehrenberg,WB;Bohlen,P;Ling,N
通讯作者：
Ling,N