Fine mapping of the pond snail left-right asymmetry (chirality) locus using RAD-Seq and fibre-FISH.
Fine mapping of the pond snail left-right asymmetry (chirality) locus using RAD-Seq and fibre-FISH.
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DOI：
10.1371/journal.pone.0071067
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Davison A
中科院分区：
文献类型：
--
作者：
Liu MM;Davey JW;Banerjee R;Han J;Yang F;Aboobaker A;Blaxter ML;Davison A
The left-right asymmetry of snails, including the direction of shell coiling, is determined by the delayed effect of a maternal gene on the chiral twist that takes place during early embryonic cell divisions. Yet, despite being a well-established classical problem, the identity of the gene and the means by which left-right asymmetry is established in snails remain unknown. We here demonstrate the power of new genomic approaches for identification of the chirality gene, “D”. First, heterozygous (Dd) pond snails Lymnaea stagnalis were self-fertilised or backcrossed, and the genotype of more than six thousand offspring inferred, either dextral (DD/Dd) or sinistral (dd). Then, twenty of the offspring were used for Restriction-site-Associated DNA Sequencing (RAD-Seq) to identify anonymous molecular markers that are linked to the chirality locus. A local genetic map was constructed by genotyping three flanking markers in over three thousand snails. The three markers lie either side of the chirality locus, with one very tightly linked (<0.1 cM). Finally, bacterial artificial chromosomes (BACs) were isolated that contained the three loci. Fluorescent in situ hybridization (FISH) of pachytene cells showed that the three BACs tightly cluster on the same bivalent chromosome. Fibre-FISH identified a region of greater that ∼0.4 Mb between two BAC clone markers that must contain D. This work therefore establishes the resources for molecular identification of the chirality gene and the variation that underpins sinistral and dextral coiling. More generally, the results also show that combining genomic technologies, such as RAD-Seq and high resolution FISH, is a robust approach for mapping key loci in non-model systems.
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DOI：
10.1159/000133632
发表时间：
1994-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
FIDLEROVA, H;SENGER, G;SHEER, D
通讯作者：
SHEER, D
影响因子：
3.7
作者：
Baird NA;Etter PD;Atwood TS;Currey MC;Shiver AL;Lewis ZA;Selker EU;Cresko WA;Johnson EA
通讯作者：
Johnson EA
影响因子：
3.7
作者：
Baxter SW;Davey JW;Johnston JS;Shelton AM;Heckel DG;Jiggins CD;Blaxter ML
通讯作者：
Blaxter ML
影响因子：
64.8
作者：
Kuroda, Reiko;Endo, Bunshiro;Shimizu, Miho
通讯作者：
Shimizu, Miho
影响因子：
3.3
作者：
Davison, Angus;Frend, Hayley T.;Eichhorn, Markus P.
通讯作者：
Eichhorn, Markus P.