Anti-fungal properties of Puroindoline A against Aspergillus glaucus
Anti-fungal properties of Puroindoline A against Aspergillus glaucus
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嘌呤吲哚啉 A 对灰绿曲霉的抗真菌特性
DOI：
10.1016/j.gaost.2020.01.002
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Yuansen Hu
中科院分区：
文献类型：
--
作者：
Wenjing Yuan;Pingping Tian;Ang Lyu;Yangyong Lyu;Wei Zhang;Shan Wei;Yuansen Hu
青绿曲霉生长在低水分的粮食中，是造成农产品损失的主要真菌之一。嘌呤多啉A （PINA）是一种富含色氨酸的碱性脂联素，能有效抑制多种植物细菌和真菌。然而，PINA抗sta的作用机制尚不清楚。glaucusremains不清楚。我们发现重组PINA （rPINA）可以抑制a。盐还原琼脂（SCDA）培养基上青丝菌丝的生长和孢子在还原琼脂（CD）培养基上的萌发。扫描电镜显示两者的形态特征不完整。rPINA处理后出现青丝孢子和菌丝。激光扫描共聚焦显微镜（LSCM）显示，rPINA可以进入孢子内部。流式细胞术和碘化丙啶（PI）染色显示，孢子膜损伤严重，特别是在0.9 mg/mL rPINA处理12 h后，膜完好的孢子减少了30.7%。此外，rPINA通过5,5 ',6,6 ' -四氯-1,1 ',3,3 ' -四乙基-吡虫啉碘化（JC-1）染色降低线粒体膜电位（MMP），并通过4 '，6-二氨基-2-苯基吲哚（DAPI）染色引起DNA损伤。上述结果表明，rPINA可能破坏青光眼的细胞膜和DNA结构，降低MMP，从而抑制青光眼的生长。本研究证实了其抗真菌作用机制，结果表明，rPINA在农业工业中具有预防采后损失的应用潜力。
Aspergillus glaucuscan grow in low moisture grain, and is one of the main fungi responsible for agricultural product losses. Puroindoline A (PINA) is a tryptophan-rich alkaline adiponectin that can effectively inhibit numerous plant bacteria and fungi. However, the mechanism of PINA againstA.glaucusremains unclear. Herein, we found that recombinant PINA (rPINA) could inhibitA.glaucusmycelia growth on salt Czapek dox agar (SCDA) medium and spore germination on Czapek dox (CD) medium. Scanning electron microscopy revealed that incomplete morphological characteristics of bothA.glaucusspores and mycelia occurred following rPINA treatment. Laser scanning confocal microscopy (LSCM) showed that rPINA could enter the interior of spores. Flow cytometry and propidium iodide (PI) staining illustrated membranes of spores were severely damaged, especially after treatment with 0.9 mg/mL rPINA for 12 h, and spores with intact membranes were reduced by 30.7%. Additionally, rPINA reduced the mitochondrial membrane potential (MMP) by 5,5′,6,6′-tetrachlorr-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) staining, and caused DNA damage by 4′,6-diamidino-2-phenylindole (DAPI) staining. These results indicated that rPINA may damage cell membranes and DNA structure and reduce MMP, thereby inhibiting the growth ofA.glaucus. The antifungal mechanism has been demonstrated in this study, and results show that rPINA has application potential in preventing postharvest loss in the agricultural industry.
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