Suppressed recombination and a pairing anomaly on the mating-type chromosome of Neurospora tetrasperma.

Suppressed recombination and a pairing anomaly on the mating-type chromosome of Neurospora tetrasperma.
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四子脉脉孢菌交配型染色体上的重组被抑制和配对异常。

DOI:
10.1093/genetics/154.2.623
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发表时间:
2000
期刊:
影响因子:
3.3
通讯作者:
Natvig,DO
Natvig,DO
中科院分区:
生物学2区
文献类型:
--
作者:
Gallegos,A;Jacobson,DJ;Raju,NB;Skupski,MP;Natvig,DO

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粗糙脉孢菌和相关的异宗配合子囊菌每孢子囊产生8个同核自不育子囊孢子。与此相反,N.四分孢子含有四个自能生殖的子囊孢子,每个子囊孢子具有两种交配类型(matA和mata)的核。N.四精子是由交配型的第一次分裂分离和第二次减数分裂和随后的有丝分裂时核纺锤体重叠引起的。最近,Merinoet等人提出了群体遗传学证据,证明在N.四分孢子,从而防止交配型的二次分裂分离和自交不育的子囊孢子的形成。本研究通过检测野生品系杂交中标记的分离,实验证实了交配型染色体的一个大片段的抑制杂交。令人惊讶的是,我们的研究还揭示了左臂上的一个区域,在那里重组是必须的。在细胞学研究中,我们证明了抑制重组与粗线期广泛的未配对区域相关。两者合计,这些结果表明一个未配对的区域相邻的一个或多个配对的区域,类似于动物性染色体的非配对和假常染色体区域。观察到的配对和专性交换可能反映了确保染色体分离的机制。
Neurospora crassa and related heterothallic ascomycetes produce eight homokaryotic self-sterile ascospores per ascus. In contrast, asci of N. tetrasperma contain four self-fertile ascospores each with nuclei of both mating types (matA and mata). The self-fertile ascospores of N. tetrasperma result from first-division segregation of mating type and nuclear spindle overlap at the second meiotic division and at a subsequent mitotic division. Recently,Merinoet al. presented population-genetic evidence that crossing over is suppressed on the mating-type chromosome of N. tetrasperma, thereby preventing second-division segregation of mating type and the formation of self-sterile ascospores. The present study experimentally confirmed suppressed crossing over for a large segment of the mating-type chromosome by examining segregation of markers in crosses of wild strains. Surprisingly, our study also revealed a region on the far left arm where recombination is obligatory. In cytological studies, we demonstrated that suppressed recombination correlates with an extensive unpaired region at pachytene. Taken together, these results suggest an unpaired region adjacent to one or more paired regions, analogous to the nonpairing and pseudoautosomal regions of animal sex chromosomes. The observed pairing and obligate crossover likely reflect mechanisms to ensure chromosome disjunction.
DOI: 10.1021/acs.orglett.7b00494
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发表时间: 1996
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