l-α, ω-Diamino acids facilitate GABA induction of larval metamorphosis in a gastropod mollusc (Haliotis rufescens)
l-α, ω-Diamino acids facilitate GABA induction of larval metamorphosis in a gastropod mollusc (Haliotis rufescens)
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l-α, ω-二氨基酸促进 GABA 诱导腹足类软体动物 (Haliotis rufescens) 幼虫变态
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
D. Morse
中科院分区：
文献类型：
--
作者：
H. Trapido;D. Morse
1. l-α，ω-二氨基酸及其衍生物促进海洋腹足动物红鲍（Haliotis rufescens）幼虫的化学诱导沉降和变态。2.本研究中使用的沉降和变态的有机诱导剂包括γ-氨基丁酸（GABA）、蝇蕈醇和天然招募甲壳红鲍（Lithothamarium californicum）的无细胞提取物的大分子部分。当诱导化合物为GABA、蝇蕈醇或L.在californicum中，二氨基酸促进剂的存在将S形浓度-响应曲线转化为双曲线，并将曲线向左移动（图2和3）。1、3和4）。在没有促进剂的情况下，希尔系数揭示了GABA诱导的显著正协同性;促进剂浓度的增加逐渐降低希尔系数（图2）。当诱导作用被钾离子直接激活时，促进剂的作用明显减弱。4.许多l-α，ω-二氨基酸（包括l-α，β-二氨基丙酸（l-DAPA）、l-α，γ-二氨基丁酸（l-DABA）、l-鸟氨酸和l-赖氨酸）在微摩尔浓度下都能促进幼虫的附着和变态诱导。在测试的许多其他氨基酸及其衍生物中，只有l-赖氨酸衍生物聚-l-赖氨酸和l-（−）-α-氨基-ε-己内酰胺作为促进剂具有活性。促进活性依赖于二氨基酸的氨基和羧基（表1）;立体化学构型也是这种活性所必需的（图7）. 5.苯二氮卓类药物泮、地西泮和氟硝西泮是某些哺乳动物GABA能神经元中GABA结合的促进剂，二氨基酸-赖氨酸不能促进本身不能诱导幼虫定居的物质的诱导（图1A和1B）。8，9）。7.本研究结果表明，控制幼虫变态的化学感受器的别构调节。
Summary1.l-α, ω-diamino acids and their derivatives facilitate the chemically induced settlement and metamorphosis of larvae ofHaliotis rufescens, a marine gastropod.2.Organic inducers of settlement and metamorphosis used in this study include γ-aminobutyric acid (GABA), muscimol, and the macromolecular fraction of a cell-free extract of the naturally recruiting crustose red alga,Lithothamnium californicum. An inorganic inducer of settlement and metamorphosis, ionic potassium, is also used.3.When the inducing compound is GABA, muscimol, or a macromolecular component ofL. californicum, the presence of a diamino acid facilitator converts a sigmoid concentration-response curve to a hyperbolic one, and shifts the curve to the left (Figs. 1, 3 and 4). The Hill coefficient in the absence of facilitator reveals significant positive cooperativity for induction by GABA; increasing concentration of facilitator progressively lowers the Hill coefficient (Fig. 2). When induction is activated directly by ionic potassium (Fig. 5), facilitators have significantly less effect.4.A number ofl-α, ω-diamino acids (includingl-α, β-diaminopropionic acid (l-DAPA),l-α, γ-diaminobutyric acid (l-DABA),l-ornithine, andl-lysine) all facilitate the induction of larval settlement and metamorphosis at micromolar concentrations (Fig. 6). Of a number of other amino acids and their derivatives tested, only thel-lysine derivatives poly-l-lysine andl-(−)-α-amino-ε-caprolactam are active as facilitators. Facilitatory activity is dependent on both of the amino groups, and the carboxyl group, of the diamino acids (Table 1); thel-stereochemical configuration also is essential for this activity (Fig. 7).5.The benzodiazepines flurazepam, diazepam, and flunitrazepam, facilitators of GABA binding in certain mammalian GABAergic neurons, do not facilitate induction of larval settlement and metamorphosis by GABA.6.The diamino acidl-lysine does not facilitate induction by substances that can not themselves induce settlement (Figs. 8, 9).7.Allosteric regulation of chemosensory receptors controlling larval metamorphosis is suggested by the results of this study.
药物-受体相互作用的定量分析：I.动力学和平衡特性的测定。
影响因子：
6.1
作者：
Weiland,GA;Molinoff,PB
通讯作者：
Molinoff,PB
影响因子：
56.9
作者：
KANDEL, ER;SCHWARTZ, JH
通讯作者：
SCHWARTZ, JH