Construction, Characterization, and Application of an Ammonium Transporter (AmtB) Deletion Mutant of the Nitrogen-Fixing Bacterium Kosakonia radicincitans GXGL-4A in Cucumis sativus L. Seedlings
Construction, Characterization, and Application of an Ammonium Transporter (AmtB) Deletion Mutant of the Nitrogen-Fixing Bacterium Kosakonia radicincitans GXGL-4A in Cucumis sativus L. Seedlings
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黄瓜幼苗中固氮细菌 Kosakonia radicincitans GXGL-4A 的铵转运蛋白 (AmtB) 缺失突变体的构建、表征和应用
DOI：
10.1007/s00284-022-03160-5
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发表时间：
2023-01
影响因子：
2.6
通讯作者：
Chen Yun-Peng
中科院分区：
文献类型：
--
作者：
Bao Yu-Qing;Zhang Meng-Ting;Feng Bao-Yun;Jieensi Wulale;Xu Yu;Xu Lu-Rong;Han Ying-Ying;Chen Yun-Peng
氮是影响作物产量的重要因素，但化学氮肥的过量使用造成了氮素利用率下降和土壤水污染。通过生物固氮减少化学氮肥的利用是实现作物绿色生产的可行方法。然而，关于如何使固氮细菌（NFB）产生更多的氨在细胞外流动的报道很少。本研究删除了NFB菌株kosakonia radicincitansGXGL-4A基因组中编码铵转运体（AmtB）的AmtB基因，并对△AmtB突变体进行了表征。结果表明，amtb基因的缺失对细菌细胞的生长没有影响。△amtB突变体在无氮培养条件下的胞外铵态氮（NH4+）含量显著高于野生型菌株GXGL-4A (WT-GXGL-4A)，表明NH4+转运被破坏。同时，利用△amtB突变体细胞进行施肥，观察了其对黄瓜幼苗生长的促进作用。NFB施肥持续提高黄瓜根际土壤ph值，△amtB处理组土壤硝态氮（NO3−）含量短期内显著高于WT-GXGL-4A处理组，但各组土壤NH4+含量无显著差异。在45 d的评价期内，土壤酶活性发生了变化，说明施用△amtB影响了黄瓜根际土壤氮循环。研究结果将为NFB GXGL-4A高效生物肥料的开发提供坚实的基础。
Nitrogen is an important factor affecting crop yield, but excessive use of chemical nitrogen fertilizer has caused decline in nitrogen utilization and soil and water pollution. Reducing the utilization of chemical nitrogen fertilizers by biological nitrogen fixation (BNF) is feasible for green production of crops. However, there are few reports on how to have more ammonium produced by nitrogen-fixing bacteria (NFB) flow outside the cell. In the present study, theamtB gene encoding an ammonium transporter (AmtB) in the genome of NFB strainKosakonia radicincitansGXGL-4A was deleted and the △amtB mutant was characterized. The results showed that deletion of theamtB gene had no influence on the growth of bacterial cells. The extracellular ammonium nitrogen (NH4+) content of the △amtB mutant under nitrogen-free culture conditions was significantly higher than that of the wild-type strain GXGL-4A (WT-GXGL-4A), suggesting disruption of NH4+transport. Meanwhile, the plant growth-promoting effect in cucumber seedlings was visualized after fertilization using cells of the △amtB mutant. NFB fertilization continuously increased the cucumber rhizosphere soil pH. The nitrate nitrogen (NO3−) content in soil in the △amtB treatment group was significantly higher than that in the WT-GXGL-4A treatment group in the short term but there was no difference in soil NH4+contents between groups. Soil enzymatic activities varied during a 45-day assessment period, indicating that △amtB fertilization influenced soil nitrogen cycling in the cucumber rhizosphere. The results will provide a solid foundation for developing the NFB GXGL-4A into an efficient biofertilizer agent.
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影响因子：
2.1
作者：
A. T. M. A. Choudhury-A.-T.-M.-A.-Choudhury-68975844;M. Kecskés;I. Kennedy
通讯作者：
A. T. M. A. Choudhury-A.-T.-M.-A.-Choudhury-68975844;M. Kecskés;I. Kennedy
影响因子：
4.6
作者：
Wang R;Zhang H;Sun L;Qi G;Chen S;Zhao X
通讯作者：
Zhao X
影响因子：
3.2
作者：
D. Meletzus;P. Rudnick;N. Doetsch;A. Green;C. Kennedy
通讯作者：
D. Meletzus;P. Rudnick;N. Doetsch;A. Green;C. Kennedy
DOI：
10.1016/b978-0-12-384939-7.10005-5
发表时间：
2012-02-29
期刊：
Principles of Molecular Virology
影响因子：
--
作者：
Cann AJ
通讯作者：
Cann AJ
油菜素类固醇诱导的二氧化碳同化与黄瓜植物叶绿体中氧化还原敏感光合酶的稳定性增加有关
DOI：
10.1016/j.bbrc.2012.08.100
发表时间：
2012-09-28
影响因子：
3.1
作者：
Jiang, Yu Ping;Cheng, Fei;Yu, Jing Quan
通讯作者：
Yu, Jing Quan