Plasma and serum lipidomics of healthy white adults shows characteristic profiles by subjects' gender and age.
Plasma and serum lipidomics of healthy white adults shows characteristic profiles by subjects' gender and age.
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DOI：
10.1371/journal.pone.0091806
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Saito Y
中科院分区：
文献类型：
--
作者：
Ishikawa M;Maekawa K;Saito K;Senoo Y;Urata M;Murayama M;Tajima Y;Kumagai Y;Saito Y
血液是发现生物标志物的常用生物流体。虽然血脂代谢产物被认为是潜在的生物标志物候选物，但它们的基本特性尚未得到很好的表征。我们的目的是(1)研究可能更适合于脂质生物标志物探索的基质类型（血清和血浆），(2)阐明脂质代谢物水平的年龄和性别相关差异，(3)在反复冻融循环的基质样品中检测脂质代谢物的稳定性。使用液相色谱-质谱法，我们对四组（15名受试者/组）的空腹血浆和血清样本（分别为25-34岁和55-64岁）的男性和女性，以及另外一组来自年轻男性的样本进行了脂质组学分析，这些样本经过反复的冻融循环。血清中溶血磷脂酰胆碱和二酰基甘油水平高于血浆样品，表明凝血过程影响血清脂质代谢物水平。与性别相关的差异突出表明，无论年龄和基质（血浆和血清）如何，许多鞘磷脂种类的水平在女性中明显高于男性。与年龄相关的差异在女性中比在男性中更为突出，并且在两种基质中，许多甘油三酯的水平在老年女性中明显高于年轻女性。冻融后血浆和血清中大多数脂质代谢物水平降低。我们的研究结果表明，血浆是探索脂质生物标志物的最佳基质，因为它代表了个体血液样本的原始特性。此外，健康成人某些血脂种类的水平存在性别和年龄相关的差异；因此，在生物标志物的探索及其在诊断中的应用中应考虑到这一点。我们关于测量血脂代谢物的样品选择和处理程序的基本发现对于确保鉴定的生物标志物的质量及其鉴定过程是重要的。
Blood is a commonly used biofluid for biomarker discovery. Although blood lipid metabolites are considered to be potential biomarker candidates, their fundamental properties are not well characterized. We aimed to (1) investigate the matrix type (serum vs. plasma) that may be preferable for lipid biomarker exploration, (2) elucidate age- and gender-associated differences in lipid metabolite levels, and (3) examine the stability of lipid metabolites in matrix samples subjected to repeated freeze-thaw cycles. Using liquid chromatography-mass spectrometry, we performed lipidomic analyses for fasting plasma and serum samples for four groups (15 subjects/group) of young and elderly (25–34 and 55–64 years old, respectively) males and females and for an additional aliquot of samples from young males, which were subjected to repeated freeze-thaw cycles. Lysophosphatidylcholine and diacylglycerol levels were higher in serum than in plasma samples, suggesting that the clotting process influences serum lipid metabolite levels. Gender-associated differences highlighted that the levels of many sphingomyelin species were significantly higher in females than in males, irrespective of age and matrix (plasma and serum). Age-associated differences were more prominent in females than in males, and in both matrices, levels of many triacylglycerols were significantly higher in elderly females than in young females. Plasma and serum levels of most lipid metabolites were reduced by freeze-thawing. Our results indicate that plasma is an optimal matrix for exploring lipid biomarkers because it represents the original properties of an individual’s blood sample. In addition, the levels of some blood lipid species of healthy adults showed gender- and age-associated differences; thus, this should be considered during biomarker exploration and its application in diagnostics. Our fundamental findings on sample selection and handling procedures for measuring blood lipid metabolites is important for ensuring the quality of biomarkers identified and its qualification process.
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DOI：
10.1073/pnas.1011278107
发表时间：
2010-09-28
影响因子：
11.1
作者：
Liu, Jun-Yan;Li, Ning;Hammock, Bruce D.
通讯作者：
Hammock, Bruce D.
影响因子：
5.5
作者：
Ben-Amor, N;Redondo, PC;Rosado, JA
通讯作者：
Rosado, JA
影响因子：
7
作者：
Ono, Masaya;Shitashige, Miki;Yamada, Tesshi
通讯作者：
Yamada, Tesshi
影响因子：
2.9
作者：
Liu, Linsheng;Aa, Jiye;Wu, Zimei
通讯作者：
Wu, Zimei
影响因子：
2.6
作者：
Sangkuhl K;Shuldiner AR;Klein TE;Altman RB
通讯作者：
Altman RB