Divergent gene expression among phytoplankton taxa in response to upwelling
Divergent gene expression among phytoplankton taxa in response to upwelling
复制标题
浮游植物类群响应上升流的不同基因表达
DOI：
10.1111/1462-2920.14361
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
A. Marchetti
中科院分区：
文献类型：
--
作者：
Robert H. Lampe;N. Cohen;Kelsey A. Ellis;K. Bruland;M. T. Maldonado;T. D. Peterson;Claire P. Till;M. Brzezinski;S. Bargu;K. Thamatrakoln;F. Kuzminov;B. Twining;A. Marchetti
浮游植物频繁大量繁殖发生在沿海涌升区，造成海洋生物生产力的热点。当寒冷、富含营养的水从深处被带到阳光照射的层时，浮游植物也被向上运送到种子表面，这些种子表面通常由硅藻控制。浮游植物对这一过程的生理反应，通常被称为上移，其特征是快速生长率和硝酸盐同化的增加。为了研究这一现象背后的分子基础，元转录组学被应用到一个模拟的上升流实验，使用天然浮游植物群落从加州上升流区。硅藻生长的增加，经过5天的孵化是由于属角毛藻和伪菱形藻。在这里，我们表明，某些水华形成硅藻表现出独特的转录反应，协调移位上升，硅藻表现出最大的转录变化后上升流，但是，共表达基因的比较暴露的不同套内的每个占主导地位的浮游植物群体的代表性过高。分析表明，硅藻frontload基因参与氮同化可能是为了在上升流事件中竞争其他群体的有效氮。我们推测，硅藻的进化成功可能是由于，在某种程度上，这种积极主动的反应，经常遇到的变化，他们的环境。
Frequent blooms of phytoplankton occur in coastal upwelling zones creating hotspots of biological productivity in the ocean. As cold, nutrient-rich water is brought up to sunlit layers from depth, phytoplankton are also transported upwards to seed surface blooms that are often dominated by diatoms. The physiological response of phytoplankton to this process, commonly referred to as shift-up, is characterized by rapid growth rates and increases in nitrate assimilation. To examine the molecular underpinnings behind this phenomenon, metatranscriptomics was applied to a simulated upwelling experiment using natural phytoplankton communities from the California Upwelling Zone. An increase in diatom growth following five days of incubation was attributed to the genera Chaetoceros and Pseudo-nitzschia. Here we show that certain bloom-forming diatoms exhibit a distinct transcriptional response that coordinates shift-up where diatoms exhibited the greatest transcriptional change following upwelling; however, comparison of coexpressed genes exposed overrepresentation of distinct sets within each of the dominant phytoplankton groups. The analysis revealed that diatoms frontload genes involved in nitrogen assimilation likely in order to outcompete other groups for available nitrogen during upwelling events. We speculate that the evolutionary success of diatoms may be due, in part, to this proactive response to frequently encountered changes in their environment.
影响因子：
3.3
作者：
Berry, David B.;Gasch, Audrey P.
通讯作者：
Gasch, Audrey P.