Mechanism of microtubule array expansion in the cytokinetic phragmoplast.
Mechanism of microtubule array expansion in the cytokinetic phragmoplast.
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DOI：
10.1038/ncomms2967
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发表时间：
2013
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
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在陆地植物中，细胞板在胞质分裂时将子细胞分开。细胞板最初在子核之间形成，并离心膨胀直至到达质膜。细胞板的离心发育是由成膜体微管阵列的离心膨胀驱动的，但这种膨胀的分子机制尚不清楚。在这里，我们表明，成膜体阵列包括稳定的微管束和动态微管。我们发现，动态微管是由稳定束上的γ-微管蛋白成核的。动态微管在正端伸长，并优先在成膜体的前缘形成新的纤维束。与此同时，它们从细胞板上移开，保持负端的限制性分布。我们认为，γ-微管蛋白复合物附着在微管束上，微管成核和成束，伴随着负端定向运动的循环，驱动成膜体的离心发育。 植物细胞分裂是由成膜体的扩张驱动的，成膜体是在胞质分裂期间在植物细胞中间形成的特征结构。Murata等人使用遗传和细胞成像方法来阐明导致成膜体扩增的微管行为。
In land plants, the cell plate partitions the daughter cells at cytokinesis. The cell plate initially forms between daughter nuclei and expands centrifugally until reaching the plasma membrane. The centrifugal development of the cell plate is driven by the centrifugal expansion of the phragmoplast microtubule array, but the molecular mechanism underlying this expansion is unknown. Here, we show that the phragmoplast array comprises stable microtubule bundles and dynamic microtubules. We find that the dynamic microtubules are nucleated by γ-tubulin on stable bundles. The dynamic microtubules elongate at the plus ends and form new bundles preferentially at the leading edge of the phragmoplast. At the same time, they are moved away from the cell plate, maintaining a restricted distribution of minus ends. We propose that cycles of attachment of γ-tubulin complexes onto the microtubule bundles, microtubule nucleation and bundling, accompanied by minus-end-directed motility, drive the centrifugal development of the phragmoplast. Plant cell division is driven by the expansion of the phragmoplast, a characteristic structure that forms in the middle of the plant cell during cytokinesis. Murata et al. use genetic and cell imaging approaches to clarify the microtubule behaviour that leads to phragmoplast expansion.
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