The Import Route of ADP/ATP Carrier into Mitochondria Separates from the General Import Pathway of Cleavable Preproteins at thetrans Side of the Outer Membrane*
The Import Route of ADP/ATP Carrier into Mitochondria Separates from the General Import Pathway of Cleavable Preproteins at thetrans Side of the Outer Membrane*
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ADP/ATP 载体进入线粒体的输入途径与外膜反侧可切割前蛋白的一般输入途径不同*
DOI：
10.1074/jbc.273.26.16374
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
A. Hönlinger
中科院分区：
文献类型：
--
作者：
M. Kübrich;J. Rassow;W. Voos;N. Pfanner;A. Hönlinger
The ADP/ATP carrier (AAC) of the mitochondrial inner membrane is synthesized in the cytosol without a cleavable presequence. The preprotein preferentially binds to the mitochondrial surface receptor Tom70 and joins the import pathway of presequence-carrying preproteins at the cis side of the outer membrane. Little is known about the translocation of the AAC across the outer membrane and where its import route separates from that of cleavable preproteins. Here we have characterized a translocation intermediate of AAC during transfer across the outer membrane. The major portion of the preprotein is exposed to the intermembrane space, while a short segment is still accessible to externally added protease. This intermediate can be quantitatively chased to the fully imported form in the inner membrane. Its accumulation depends on Tom7, but not on the intermembrane space domain of Tom22 in contrast to cleavable preproteins. Moreover, opening of the intermembrane space inhibits the import of AAC, but not that of cleavable preproteins into mitoplasts. We conclude that the import route of AAC diverges from the general import pathway of cleavable preproteins already at the trans side of the outer membrane.
影响因子：
11.4
作者：
HINES, V;BRANDT, A;SCHATZ, G
通讯作者：
SCHATZ, G
ATP 在细胞色素 c1 和腺嘌呤核苷酸易位子线粒体内分选中的作用。
DOI：
10.1002/j.1460-2075.1992.tb05584.x
发表时间：
1992
期刊：
The EMBO journal
影响因子：
--
作者：
Wachter,C;Schatz,G;Glick,BS
通讯作者：
Glick,BS