A cell culture system for the structure and hydrogel properties of basement membranes; Application to capillary walls.
A cell culture system for the structure and hydrogel properties of basement membranes; Application to capillary walls.
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一个细胞培养系统，用于基底膜的结构和水凝胶特性；应用于毛细管墙。
DOI：
10.1007/s12195-012-0221-3
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Calabro, Anthony
中科院分区：
文献类型：
--
作者：
Bruggeman, Leslie A.;Doan, Ryan P.;Loftis, Jacqueline;Darr, Aniq;Calabro, Anthony
在特殊的毛细血管床中，如肾小球，基底膜的片状结构与相反的内皮和上皮单层结合形成肾脏的功能过滤单元。利用一种新颖的交联方法在胶原基质上，我们创造了一种新的水凝胶支架来代替基膜。采用简单的浇铸法制备1-5μm的水凝胶支架薄膜，支架适合长期静态培养，具有与标准I型胶原基质相似的细胞附着和长期细胞活力。评估了水凝胶支架的体积扩散和蛋白质渗透性，并将其用于灌注室，在灌注室中，它可以承受肾小球毛细血管的典型水压。因此，该系统为肾上皮足细胞和内皮细胞的共培养提供了合适的细胞底物，该装置复制了两种细胞类型在体内并置的几何形状，与它们的基膜有关。
In specialized capillary beds such as the kidney glomerulus, the sheet-like structure of the basement membrane in conjunction with opposing monolayers of endothelium and epithelium form the functioning filtration unit of the kidney. Using a novel cross-linking method on a collagen substrate, we have created a novel hydrogel scaffold to substitute for the basement membrane. Using a simple casting method to create thin films of the hydrogel scaffold (1–5μm), the scaffolds were suitable for long-term static culture, and supported cell attachment and long term cell viability similar to a standard type I collagen substrate. Bulk diffusion and protein permeability of the hydrogel scaffold were evaluated, in addition to its use in a perfusion chamber where it withstood hydraulic pressures typical for glomerular capillaries. This system thus provided a suitable cell substrate for the co-culture of renal epithelial podocytes and endothelial cells in a device that replicates the geometry of the in vivo juxtaposition of the two cell types in relation to their basement membrane.
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影响因子：
19.6
作者：
SCHNAPER, HW;KLEINMAN, HK;GRANT, DS
通讯作者：
GRANT, DS
DOI：
10.1126/science.1188302
发表时间：
2010-06-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Huh D;Matthews BD;Mammoto A;Montoya-Zavala M;Hsin HY;Ingber DE
通讯作者：
Ingber DE
影响因子：
--
作者：
Ballermann, Barbara J.
通讯作者：
Ballermann, Barbara J.
影响因子：
19.6
作者：
Akis, N;Madaio, MP
通讯作者：
Madaio, MP
影响因子：
13.6
作者：
Schwartz, EJ;Cara, A;Klotman, PE
通讯作者：
Klotman, PE