X-ray diffraction studies of the cross-bridge intermediate states.
X-ray diffraction studies of the cross-bridge intermediate states.
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跨桥中间态的 X 射线衍射研究。
DOI：
10.1007/978-1-4684-6039-1_32
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发表时间：
1998
影响因子：
--
通讯作者：
Yu,LC
中科院分区：
文献类型：
--
作者：
Xu,S;Malinchik,S;Frisbie,S;Gu,J;Kraft,T;Rapp,G;Chalovich,JM;Brenner,B;Yu,LC
从剥皮的兔腰肌纤维获得二维 X 射线衍射。目标是通过使用核苷酸及其类似物将跨桥群体的结构与跨桥循环中的各种中间状态相关联。研究发现，在含有 ATP 的溶液中的松弛肌肉中，跨桥以平衡状态分布在三个群体中：那些与肌动蛋白分离并在肌球蛋白螺旋上有序的、那些分离且无序的、以及那些以随机方向弱附着于肌动蛋白的。这三个种群之间的分布高度依赖于温度。那些分离但以围绕肌球蛋白主链的螺旋结构排列的物质很可能处于 M ADP P1 状态，这支持了 Wray (1987) 的早期建议。还发现，带有结合 MgADP 的连接的跨桥在结构上与没有核苷酸的跨桥不同，这与我们早期通过渗透压缩的发现之一一致（Xu 等人，1993）。另一个有趣的发现是，类似物 AMP-PNP 被发现是一种 ATP 类似物，而不是许多研究小组之前报道的 ADP 类似物。
Two dimensional x-ray diffraction was obtained from skinned rabbit psoas muscle fibers. The goal is to correlate structures of the cross-bridge population with various intermediate states in the cross-bridge cycle by using nucleotides and their analogs. It was found that in a relaxed muscle in ATP containing solutions, cross-bridges are distributed in three populations in equilibrium: those detached from actin and ordered on the myosin helix, those that are detached and disordered, and those weakly attached to actin in random orientations. The distribution among the three populations is highly dependent on temperature. Those that are detached and yet ordered in a helical structure surrounding the myosin backbone are very likely in the M ADP P1state, supporting an earlier suggestion by Wray (1987). It was also found that the attached cross-bridges with bound MgADP are structurally distinct from those without nucleotide, in agreement with one of our earlier findings by osmotic compression (Xu et al., 1993). Another finding of interest is that the analog AMP-PNP was found to be an ATP analog, rather than an ADP analog as it has been reported previously by many research groups.
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使用新型磷酸盐荧光探针测量相关兔骨骼肌动球蛋白的核苷三磷酸裂解和磷酸盐释放步骤的动力学。
DOI：
10.1021/bi970540h
发表时间：
1997
期刊：
Biochemistry.
影响因子：
--
作者：
White,HD;Belknap,B;Webb,MR
通讯作者：
Webb,MR
在钒酸盐存在下对单个青蛙皮肌纤维进行的 X 射线衍射研究。
DOI：
--
发表时间：
1995
期刊：
Journal of Biochemistry (Tokyo)
影响因子：
--
作者：
S. Takemori;M. Yamaguchi;N. Yagi
通讯作者：
N. Yagi
影响因子：
5.5
作者：
BRENNER, B;YU, LC
通讯作者：
YU, LC
卡德斯蒙对骨骼肌主动力和松弛纤维刚度的并行抑制：对力产生途径的影响。
DOI：
10.1073/pnas.88.13.5739
发表时间：
1991
影响因子：
11.1
作者：
Brenner,B;Yu,LC;Chalovich,JM
通讯作者：
Chalovich,JM
影响因子：
3.4
作者：
Fajer,PG;Fajer,EA;Schoenberg,M;Thomas,DD
通讯作者：
Thomas,DD