Disruption of the homogentisate solanesyltransferase gene results in albino and dwarf phenotypes and root, trichome and stomata defects in Arabidopsis thaliana.
Disruption of the homogentisate solanesyltransferase gene results in albino and dwarf phenotypes and root, trichome and stomata defects in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0094031
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Chao Y;Kang J;Zhang T;Yang Q;Gruber MY;Sun Y
同源茄烷基转移酶(HST)是类胡萝卜素和脱落酸(ABA)生物合成途径中的电子受体，在PQ生物合成中起重要作用。我们分离并鉴定了一个HST基因的T-DNA插入突变体，该突变体表现出白化和矮化表型。聚合酶链式反应分析和功能互补也证实了突变表型是由HST基因的破坏引起的。突变体还存在一些发育缺陷，包括毛状体发育和气孔关闭缺陷。叶绿体发育也受阻，几乎没有叶绿素(Chl)。叶绿体的发育缺陷与SDS-PAGE结果和RNAi转基因表型一致。外源赤霉素(GA)可以部分挽救矮秆表型和根系发育缺陷，外源ABA可以挽救气孔关闭缺陷。进一步的分析表明，在pds2-1突变体中ABA和GA的水平都很低，这表明GAs和ABA对生物合成的抑制是导致pds2-1突变体的表型的原因之一。在pds2-1突变体中发现了早花表型，这表明hst基因的缺失可能通过部分调节植物激素来促进开花。RNA测序表明，HST基因的中断导致了许多与开花时间调控以及PQ、Chl、GAs、ABA和类胡萝卜素的生物合成有关的基因的表达变化。这些结果表明，HST在叶绿体发育、激素生物合成、色素积累和植物发育中是必需的。
Homogentisate solanesyltransferase (HST) plays an important role in plastoquinone (PQ) biosynthesis and acts as the electron acceptor in the carotenoids and abscisic acid (ABA) biosynthesis pathways. We isolated and identified a T-DNA insertion mutant of the HST gene that displayed the albino and dwarf phenotypes. PCR analyses and functional complementation also confirmed that the mutant phenotypes were caused by disruption of the HST gene. The mutants also had some developmental defects, including trichome development and stomata closure defects. Chloroplast development was also arrested and chlorophyll (Chl) was almost absent. Developmental defects in the chloroplasts were consistent with the SDS-PAGE result and the RNAi transgenic phenotype. Exogenous gibberellin (GA) could partially rescue the dwarf phenotype and the root development defects and exogenous ABA could rescue the stomata closure defects. Further analysis showed that ABA and GA levels were both very low in the pds2-1 mutants, which suggested that biosynthesis inhibition by GAs and ABA contributed to the pds2-1 mutants' phenotypes. An early flowering phenotype was found in pds2-1 mutants, which showed that disruption of the HST gene promoted flowering by partially regulating plant hormones. RNA-sequencing showed that disruption of the HST gene resulted in expression changes to many of the genes involved in flowering time regulation and in the biosynthesis of PQ, Chl, GAs, ABA and carotenoids. These results suggest that HST is essential for chloroplast development, hormone biosynthesis, pigment accumulation and plant development.
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影响因子：
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