Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots.
Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots.
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外源钙对黄瓜根缺氧反应蛋白影响的蛋白质组学分析
DOI：
10.1186/1477-5956-10-42
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发表时间：
2012-07-12
期刊：
影响因子：
2
通讯作者：
Guo S
中科院分区：
文献类型：
--
作者：
He L;Lu X;Tian J;Yang Y;Li B;Li J;Guo S
缺氧是一种植物胁迫因子，特别是在水培黄瓜中。钙参与胁迫信号的传递和植物的生长。目的探讨外源钙对黄瓜（Cucumis sativusL.）缺氧反应蛋白的影响。简历。采用双向电泳（2-DE）和质谱法对金春2号根进行蛋白质组学分析。结果以黄瓜为研究对象，分析了缺氧对植物生长的影响。在缺氧条件下，38个酶相关蛋白点的表达发生变化。用基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOF/TOF MS）鉴定了其中的30个斑点。蛋白质按功能基团进行分类，包括糖酵解、三羧酸（TCA）循环、发酵代谢、氮代谢、能量代谢、蛋白质合成和应激防御。外源性钙似乎通过这些代谢和生理系统来缓解缺氧应激。Western blotting检测乙醇脱氢酶（ADH）和丙酮酸脱羧酶（PDC）的积累情况；低氧条件下，钙进一步增加了ADH和PDC的表达。此外，采用半定量逆转录聚合酶链反应（RT-PCR）来评估差异表达蛋白的转录水平。结论外源钙增强了缺氧条件下植物糖酵解、TCA循环、发酵代谢、氮代谢和活性氧防御等相关酶的表达。钙似乎诱导了黄瓜幼苗的耐氧性。这些现象促使我们进一步研究黄瓜在缺氧条件下对外源钙的响应机制。
BackgroundHypoxia acts as a plant stress factor, particularly in cucumbers plants under hydroponic culture. Calcium is involved in stress signal transmission and in the growth of plants. To determine the effect of exogenous calcium on hypoxic-responsive proteins in cucumber (Cucumis sativusL. cv. Jinchun No.2) roots, proteomic analysis was performed using two-dimensional electrophoresis (2-DE) and mass spectrometry.ResultsCucumber roots were used to analyze the influence of hypoxia on plants. The expressions of 38 protein spots corresponding to enzymes were shown to change in response to hypoxia. Of these, 30 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS analysis). The proteins were categorized according to functional groups, including glycolysis, the tricarboxylic acid (TCA) cycle, fermentative metabolism, nitrogen metabolism, energy metabolism, protein synthesis and defense against stress. Exogenous calcium appeared to alleviate hypoxic stress via these metabolic and physiological systems. Western blotting was used to analyze the accumulation of alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC); calcium further increased the expression of ADH and PDC under hypoxia. In addition, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to assess the transcript levels of differentially expressed proteins.ConclusionsExogenous calcium enhanced the expression of enzymes involved in glycolysis, the TCA cycle, fermentative metabolism, nitrogen metabolism, and reactive oxygen species (ROS) defense in plants under hypoxia. Calcium appears to induce hypoxic tolerance of cucumber seedlings. These phenomena have prompted us to further investigate the mechanisms by which cucumbers respond to exogenous calcium under hypoxia.
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影响因子：
3.5
作者：
Cross, RL;Müller, V
通讯作者：
Müller, V
影响因子：
2.1
作者：
Gao, HB;Chen, GL;Lin, HA
通讯作者：
Lin, HA
影响因子：
2.9
作者：
DUNCAN, R;HERSHEY, JWB
通讯作者：
HERSHEY, JWB
盐胁迫下黄瓜幼苗根部的蛋白质组学分析
影响因子：
3.8
作者：
Du, Chang-Xia;Fan, Huai-Fu;Li, Juan
通讯作者：
Li, Juan
影响因子：
7.4
作者：
FORSTHOEFEL, NR;CUSHMAN, MAF;CUSHMAN, JC
通讯作者：
CUSHMAN, JC