Stimulatory Effect of Magnetite Nanoparticles on a Highly Enriched Butyrate-Oxidizing Consortium.
Stimulatory Effect of Magnetite Nanoparticles on a Highly Enriched Butyrate-Oxidizing Consortium.
复制标题
磁铁矿纳米颗粒对高富集丁酸盐氧化群落的刺激作用
DOI：
10.3389/fmicb.2018.01480
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Fu L;Song T;Zhang W;Zhang J;Lu Y
在产甲烷菌存在的情况下，丁酸的合养氧化被一些细菌专家分解。在本研究中，从青藏高原的湿地沉积物中获得了高度浓缩的丁酸盐氧化联合体。在浓缩的连续转移过程中，添加磁性纳米颗粒(纳米Fe3O4)持续增强丁酸盐的氧化和CH4的产生。分子分析表明，该联合体的所有细菌序列均属于合胞单胞菌，其中与沃尔菲合胞单胞菌关系最近，96%的古细菌序列与甲烷杆菌有亲缘关系，其余序列与甲烷杆菌有亲缘关系。添加石墨和碳纳米管替代纳米Fe3O4也产生了类似的刺激效应。然而，纳米Fe3O4表面的二氧化硅涂层完全消除了这种刺激作用。对一株合肥单胞菌和两株产甲烷菌的无菌培养对照试验表明，纳米Fe3O4对无菌培养无影响。综上所述，本研究的结果支持丁酸盐的同养氧化很可能是通过在导电纳米材料存在下的物种间直接电子转移来促进的。
Syntrophic oxidation of butyrate is catabolized by a few bacteria specialists in the presence of methanogens. In the present study, a highly enriched butyrate-oxidizing consortium was obtained from a wetland sediment in Tibetan Plateau. During continuous transfers of the enrichment, the addition of magnetite nanoparticles (nanoFe3O4) consistently enhanced butyrate oxidation and CH4 production. Molecular analysis revealed that all bacterial sequences from the consortium belonged to Syntrophomonas with the closest relative of Syntrophomonas wolfei and 96% of the archaeal sequences were related to Methanobacteria with the remaining sequences to Methanocella. Addition of graphite and carbon nanotubes for a replacement of nanoFe3O4 caused the similar stimulatory effect. Silica coating of nanoFe3O4 surface, however, completely eliminated the stimulatory effect. The control experiment with axenic cultivation of a Syntrophomonas strain and two methanogen strains showed no effect by nanoFe3O4. Together, the results in the present study support that syntrophic oxidation of butyrate is likely facilitated by direct interspecies electron transfer in the presence of conductive nanomaterials.
登录
查看更多内容
影响因子：
7.7
作者：
McInerney, Michael J.;Sieber, Jessica R.;Gunsalus, Robert P.
通讯作者：
Gunsalus, Robert P.
影响因子：
4.4
作者：
Gan, Yanlu;Qiu, Qiongfen;Lu, Yahai
通讯作者：
Lu, Yahai
影响因子：
4.4
作者：
Rotaru, Amelia-Elena;Shrestha, Pravin Malla;Lovley, Derek R.
通讯作者：
Lovley, Derek R.
iTRAQ 定量蛋白质组学分析揭示了磁铁矿促进丙酸盐甲烷化的途径
影响因子：
12.8
作者：
Jing, Yuhang;Wan, Jingjing;Luo, Gang
通讯作者：
Luo, Gang
磁铁矿补偿了细胞外电子交换中菌毛蛋白相关的 c 型细胞色素的缺乏
影响因子：
5.1
作者：
Liu, Fanghua;Rotaru, Amelia-Elena;Lovley, Derek R.
通讯作者：
Lovley, Derek R.