Evaluation of Oxidative Stress Levels and Antioxidant Enzyme Activities in Burst Fractures.
Evaluation of Oxidative Stress Levels and Antioxidant Enzyme Activities in Burst Fractures.
复制标题
评估爆发骨折中氧化应激水平和抗氧化剂活性。
DOI：
10.12659/msm.908312
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发表时间：
2018-01-11
期刊：
影响因子：
--
通讯作者：
Aycan A
中科院分区：
文献类型：
--
作者：
Kuyumcu F;Aycan A
脊柱爆裂性骨折是脊柱损伤时发生的病理，并因此导致显着的死亡率和发病率。脊髓损伤中的爆裂性骨折会导致快速而显着的氧化应激。严重脊髓损伤中除了原发性损伤外，后续的继发性损伤主要是由于炎症级联激活和自由基产生过多造成的。这项研究评估了爆裂骨折中的氧化应激和抗氧化酶水平。 20 名爆裂性骨折患者被诊断并接受手术，20 名健康对照受试者纳入研究。手术前后使用美国脊柱损伤协会损伤量表（ASIA）评估神经状态。神经功能评分为 ASIA A：完全缺陷，ASIA B-D：不完全缺陷，ASIA E：神经功能完整。采用分光光度法测量丙二醛 (MDA) 和低谷胱甘肽 (GSH)、谷胱甘肽过氧化物酶 (GPx) 水平（代表脂质过氧化物含量）。在患者受伤后 2 天内进行评估。爆裂性骨折组的 MDA 水平较高（p<0.001），而对照组的 GSH 和 SOD 活性较高（均 p<0.001）。各组之间的 GPx 水平没有统计学上的显着差异 (p=0.482)。氧化应激似乎与爆裂性骨折有关。考虑到爆裂性骨折在脊髓损伤中的重要性，更好地了解这些机制可能有助于确定爆裂性骨折后氧化应激的作用。前瞻性、随机、对照试验可能会揭示新的治疗方法，其中包括针对氧化应激的爆炸性骨折的抗氧化剂。
Spinal burst fractures are pathologies that occur in spinal injuries and cause significant mortality and morbidity as a result. Burst fractures in spinal cord injuries can result in rapid and significant oxidative stress. In addition to the primary injury in severe spinal cord injuries, subsequent secondary lesions are mainly due to inflammatory cascade activation and excessive production of free radicals. This study evaluated oxidative stress and antioxidant enzyme levels in burst fractures. Twenty patients with burst fractures were diagnosed and underwent surgery and 20 healthy control subjects were included in the study. Neurological status was evaluated using the American Spine Injury Association Impairment Scale (ASIA) before and after surgery. Neurological function was scored as ASIA A: complete deficits, ASIA B–D: incomplete deficits, and ASIA E: neurologically intact. Spectrophotometry was performed to measure malondialdehyde (MDA) and low glutathione (GSH), glutathione peroxidase (GPx) levels, which represent lipid peroxide content. Evaluations were performed within 2 days after injury in the patients. MDA levels were higher in the burst fracture group (p<0.001), whereas GSH and SOD activities were higher in the control group (both p<0.001). There was no statistically significant difference in GPx levels between the groups (p=0.482). Oxidative stress appears to be related to burst fractures. Considering the importance of burst fractures in spinal cord injuries, a better understanding of these mechanisms may help in defining the role of oxidative stress after burst fractures. Prospective, randomized, controlled trials may reveal new therapeutic approaches that include antioxidants for explosive fractures focusing on oxidative stress.
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影响因子：
3
作者：
Hu, R;Mustard, CA;Burns, C
通讯作者：
Burns, C
DOI：
10.1515/jbcpp-2013-0042
发表时间：
2014-02-01
影响因子：
--
作者：
Alzoghaibi, Mohammed A.;Al-Mofleh, Ibrahem A.;Al-Jebreen, Abdulrahman M.
通讯作者：
Al-Jebreen, Abdulrahman M.
影响因子：
5.9
作者：
Bensch, FV;Kiuru, MJ;Koskinen, SK
通讯作者：
Koskinen, SK
影响因子：
7.4
作者：
Antunes, F;Han, D;Cadenas, E
通讯作者：
Cadenas, E
影响因子：
4.8
作者：
Kamencic, H;Griebel, RW;Juurlink, BHJ
通讯作者：
Juurlink, BHJ