Utilization of 3D printing technology to facilitate and standardize soft tissue testing.
Utilization of 3D printing technology to facilitate and standardize soft tissue testing.
复制标题
利用3D打印技术来促进和标准化软组织测试。
DOI：
10.1038/s41598-018-29583-4
复制
发表时间：
2018-07-27
影响因子：
4.6
通讯作者：
Hammer N
中科院分区：
文献类型：
--
作者：
Scholze M;Singh A;Lozano PF;Ondruschka B;Ramezani M;Werner M;Hammer N
三维 (3D) 打印已得到广泛应用，可用于定制生物力学实验中的夹紧机构。本报告将描述我们使用 3D 打印夹具安装不同解剖区域软组织的经验。将讨论该技术的可行性和潜在局限性。组织是在新鲜状态下采购的，包括人体皮肤、韧带和肌腱。标准化夹具和固定装置经过 3D 打印并用于安装样本。在与数字图像相关和疲劳试验相结合的准静态拉伸试验中，我们表征了夹紧技术的适用性。利用扫描电子显微镜来评估样本，以评估机械测试后细胞外基质的完整性。 3D 打印夹具在准静态测试期间没有显示出与夹紧相关的故障迹象，并且在夹紧区域、过渡夹紧区域和获得应变数据的中心区域发现了完整的细胞外基质。在疲劳测试中，材料滑移率较低，允许循环测试超过 105 次。与其他夹紧技术相比，3D 打印夹具可以简化并加快样本处理，高度适应样本几何形状，非常适合软组织生物力学的高标准化和高通量实验。
Three-dimensional (3D) printing has become broadly available and can be utilized to customize clamping mechanisms in biomechanical experiments. This report will describe our experience using 3D printed clamps to mount soft tissues from different anatomical regions. The feasibility and potential limitations of the technology will be discussed. Tissues were sourced in a fresh condition, including human skin, ligaments and tendons. Standardized clamps and fixtures were 3D printed and used to mount specimens. In quasi-static tensile tests combined with digital image correlation and fatigue trials we characterized the applicability of the clamping technique. Scanning electron microscopy was utilized to evaluate the specimens to assess the integrity of the extracellular matrix following the mechanical tests. 3D printed clamps showed no signs of clamping-related failure during the quasi-static tests, and intact extracellular matrix was found in the clamping area, at the transition clamping area and the central area from where the strain data was obtained. In the fatigue tests, material slippage was low, allowing for cyclic tests beyond 105 cycles. Comparison to other clamping techniques yields that 3D printed clamps ease and expedite specimen handling, are highly adaptable to specimen geometries and ideal for high-standardization and high-throughput experiments in soft tissue biomechanics.
登录
查看更多内容
DOI：
10.1016/j.jmbbm.2016.01.018
发表时间：
2016-08-01
影响因子：
3.9
作者：
Hammer, Niels;Huster, Daniel;Scheidt, Holger A.
通讯作者：
Scheidt, Holger A.
影响因子：
2.4
作者：
Schleifenbaum, Stefan;Prietzel, Torsten;Hammer, Niels
通讯作者：
Hammer, Niels
一种新颖、廉价且易于使用的腱夹，用于体外生物力学测试
影响因子：
2.2
作者：
Shi, DuFang;Wang, DongMei;Liu, Anmin
通讯作者：
Liu, Anmin
DOI：
10.1016/j.jmbbm.2013.11.020
发表时间：
2014-02-01
影响因子：
3.9
作者：
Reyes, A. M.;Jahr, H.;Zadpoor, A. A.
通讯作者：
Zadpoor, A. A.
影响因子：
8.2
作者：
Dai Z;Ronholm J;Tian Y;Sethi B;Cao X
通讯作者：
Cao X