Multiple insecticide resistance mechanisms involving metabolic changes and insensitive target sites selected in anopheline vectors of malaria in Sri Lanka.
Multiple insecticide resistance mechanisms involving metabolic changes and insensitive target sites selected in anopheline vectors of malaria in Sri Lanka.
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斯里兰卡疟疾按蚊媒介中涉及代谢变化和不敏感靶位点的多种杀虫剂抗性机制。
DOI：
10.1186/1475-2875-7-168
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发表时间：
2008-08-28
期刊：
影响因子：
3
通讯作者：
Karunaratne, S. H. P. Parakrama
中科院分区：
文献类型：
--
作者：
Perera, M. Devika B.;Hemingway, Janet;Karunaratne, S. H. P. Parakrama
对斯里兰卡5个区（阿努拉得普勒、库鲁内加拉、莫尼拉加拉、普塔拉姆和亭可马里）主要疟疾病媒库利相按蚊和次要疟媒亚按蚊的抗药性现状和抗药性机制进行了研究。对来自阿努拉达普拉县的其他8种按蚊，即环状按蚊、巴氏按蚊、詹姆斯按蚊、尼日利亚按蚊、丁氏按蚊、特拉按蚊、迷走按蚊和瓦鲁纳按蚊也进行了检测。成年雌性暴露于WHO鉴别剂量的滴滴涕、马拉硫磷、杀虫硫磷、残杀威、氯氟氰菊酯、氯氰菊酯、溴氰菊酯、氯氰菊酯和乙醚菊酯。利用增效剂、生化和代谢技术评估了酯酶、谷胱甘肽s -转移酶（GST）和单氧酶的代谢抗性机制，以及乙酰胆碱酯酶靶点的敏感性。所有按蚊种类均有较高的DDT抗性。所有的一个。文化相与文化。除亚蚊种群对马拉硫磷有抗性外，其余种群均有抗性。库鲁内加拉的culiciifacies，那里没有马拉硫磷羧酸酯酶活性。库鲁内加拉族和普特拉姆族。菌种易受蚊虫叮咬。所有的安。culicii相种群对氨基甲酸酯敏感。两种昆虫对氯氰菊酯和氟氰菊酯的不同剂量均敏感，但对其他拟除虫菊酯的抗性程度不同。在其他8种按蚊中，只有安。尼日利亚和安。弓形虫对所有杀虫剂均有抗药性，这可能与它们大量接触农业杀虫剂有关。一个。迷走蝇对氯菊酯有一定的抗性。两者的酯酶、GSTs和单加氧酶均升高。文化相与文化。subpictus。库鲁内加拉省和亭可马里省的AChE对杀虫剂最敏感。亭可马里岛的文化相种群和高度不敏感。subpictus人口。这些田间种群中抗性分离的复杂性强调需要新的分子工具来识别基因组多样性、抗性基因的差异上调和不同结合特异性，以及具有不同媒介能力的不同亚种的存在。
The current status of insecticide resistance and the underlying resistance mechanisms were studied in the major vector of malaria, Anopheles culicifacies, and the secondary vector, Anopheles subpictus in five districts (Anuradhapura, Kurunegala, Moneragala, Puttalam and Trincomalee) of Sri Lanka. Eight other anophelines, Anopheles annularis, Anopheles barbirostris, Anopheles jamesii, Anopheles nigerrimus, Anopheles peditaeniatus, Anopheles tessellatus, Anopheles vagus and Anopheles varuna from Anuradhapura district were also tested. Adult females were exposed to the WHO discriminating dosages of DDT, malathion, fenitrothion, propoxur, λ-cyhalothrin, cyfluthrin, cypermethrin, deltamethrin, permethrin and etofenprox. The presence of metabolic resistance by esterase, glutathione S-transferase (GST) and monooxygenase-based mechanisms, and the sensitivity of the acetylcholinesterase target site were assessed using synergists, and biochemical, and metabolic techniques. All the anopheline species had high DDT resistance. All An. culicifacies and An. subpictus populations were resistant to malathion, except An. culicifacies from Kurunegala, where there was no malathion carboxylesterase activity. Kurunegala and Puttalam populations of An. culicifacies were susceptible to fenitrothion. All the An. culicifacies populations were susceptible to carbamates. Both species were susceptible to the discriminating dosages of cypermethrin and cyfluthrin, but had different levels of resistance to other pyrethroids. Of the 8 other anophelines, only An. nigerrimus and An. peditaeniatus were resistant to all the insecticides tested, probably due to their high exposure to the insecticides used in agriculture. An. vagus showed some resistance to permethrin. Esterases, GSTs and monooxygenases were elevated in both An. culicifacies and An. subpictus. AChE was most sensitive to insecticides in Kurunegala and Trincomalee An. culicifacies populations and highly insensitive in the Trincomalee An. subpictus population. The complexity of the resistance segregating in these field populations underlines the need for new molecular tools to identify the genomic diversity, differential upregulation and different binding specificities of resistance conferring genes, and the presence of different subspecies with different vectorial capacities.
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影响因子：
2.1
作者：
Amerasinghe, FP;Konradsen, F;Amerasinghe, PH
通讯作者：
Amerasinghe, PH
DOI：
10.4269/ajtmh.1999.60.421
发表时间：
1999-03-01
影响因子：
3.3
作者：
Amerasinghe, PH;Amerasinghe, FP;Wirtz, RA
通讯作者：
Wirtz, RA
影响因子：
2.1
作者：
AMERASINGHE, PH;AMERASINGHE, FP;RATHNAYAKE, AMS
通讯作者：
RATHNAYAKE, AMS
影响因子：
1.9
作者：
HERATH, PRJ;JAYAWARDENA, KGI;HARRIS, J
通讯作者：
HARRIS, J
影响因子：
4.1
作者：
O'Reilly, Andrias O.;Khambay, Bhupinder P. S.;Davies, T. G. Emyr
通讯作者：
Davies, T. G. Emyr