Disruption of an RNA-binding hinge region abolishes LHP1-mediated epigenetic repression.
Disruption of an RNA-binding hinge region abolishes LHP1-mediated epigenetic repression.
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DOI：
10.1101/gad.305227.117
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发表时间：
2017-11-01
影响因子：
10.5
通讯作者：
Dean C
中科院分区：
文献类型：
--
作者：
Berry S;Rosa S;Howard M;Bühler M;Dean C
在这项研究中，Berry等人研究了拟南芥中LIKE异染色质蛋白1 （LHP1）不同结构域的功能。他们发现LHP1在体外通过内在无序的铰链区与RNA结合，并且铰链区和H3K27me3识别都有助于LHP1的定位和H3K27me3的维持。基因抑制的表观遗传维持对发育至关重要。多梳复合体是这种记忆的核心，但潜在机制的许多方面仍不清楚。LIKE异染色质蛋白1 （LHP1）与Polycomb沉积的H3K27me3结合，在拟南芥中抑制许多Polycomb靶基因是必需的。在这里，我们表明LHP1在体外通过内在无序的铰链区与RNA结合。通过独立干扰rna结合铰链区和H3K27me3 （Lys27处组蛋白H3的三甲基化）识别，我们发现两者都促进了LHP1的定位和H3K27me3的维持。rna结合铰链区域的破坏也阻止了亚核病灶的形成，亚核病灶是表观遗传抑制的潜在重要结构。
In this study, Berry et al. investigated the functions of the different domains of LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) in Arabidopsis. They show that LHP1 binds RNA in vitro through the intrinsically disordered hinge region and show that both the hinge region and H3K27me3 recognition facilitate LHP1 localization and H3K27me3 maintenance. Epigenetic maintenance of gene repression is essential for development. Polycomb complexes are central to this memory, but many aspects of the underlying mechanism remain unclear. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) binds Polycomb-deposited H3K27me3 and is required for repression of many Polycomb target genes in Arabidopsis. Here we show that LHP1 binds RNA in vitro through the intrinsically disordered hinge region. By independently perturbing the RNA-binding hinge region and H3K27me3 (trimethylation of histone H3 at Lys27) recognition, we found that both facilitate LHP1 localization and H3K27me3 maintenance. Disruption of the RNA-binding hinge region also prevented formation of subnuclear foci, structures potentially important for epigenetic repression.
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影响因子：
16
作者：
Keller, Claudia;Adaixo, Ricardo;Buehler, Marc
通讯作者：
Buehler, Marc
影响因子：
9.3
作者：
Berry S;Dean C;Howard M
通讯作者：
Howard M
影响因子：
19
作者：
Canzio, Daniele;Larson, Adam;Narlikar, Geeta J.
通讯作者：
Narlikar, Geeta J.
影响因子：
64.8
作者：
Larson AG;Elnatan D;Keenen MM;Trnka MJ;Johnston JB;Burlingame AL;Agard DA;Redding S;Narlikar GJ
通讯作者：
Narlikar GJ
影响因子：
16.8
作者：
Keller, Claudia;Kulasegaran-Shylini, Raghavendran;Buehler, Marc
通讯作者：
Buehler, Marc