Cell-free microRNAs: potential biomarkers in need of standardized reporting.
Cell-free microRNAs: potential biomarkers in need of standardized reporting.
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DOI：
10.3389/fgene.2013.00056
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发表时间：
2013
影响因子：
3.7
通讯作者：
Reid G
中科院分区：
文献类型：
--
作者：
Kirschner MB;van Zandwijk N;Reid G
MicroRNA在多种生物流体中大量存在并且令人惊讶地稳定。这些发现之后，许多基于逆转录实时定量PCR（RT-qPCR）的报告揭示了使用体液中microRNA水平作为疾病生物标志物的临床潜力。尽管文献数量迅速增加，但该领域未能采用一套标准化标准来报告用于定量无细胞microRNA的方法。许多基于RT-qPCR的研究不仅未能解决定量实时PCR实验（MIQE）标准的最低信息发布，而且经常在样品来源和RNA分离的描述中明显缺乏细节。其直接结果是，往往无法比较不同研究的结果，这反过来又阻碍了该领域的进展。为了解决这一点，我们提出了一套简单的标准，与MIQE结合使用，以揭示无细胞microRNA作为生物标志物的真正潜力。
MicroRNAs are abundantly present and surprisingly stable in multiple biological fluids. These findings have been followed by numerous reverse transcription real-time quantitative PCR (RT-qPCR)-based reports revealing the clinical potential of using microRNA levels in body fluids as a biomarker of disease. Despite a rapidly increasing body of literature, the field has failed to adopt a set of standardized criteria for reporting the methodology used in the quantification of cell-free microRNAs. Not only do many studies based on RT-qPCR fail to address the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) criteria but frequently there is also a distinct lack of detail in descriptions of sample source and RNA isolation. As a direct result, it is often impossible to compare the results of different studies, which in turn, hinders progress in the field. To address this point, we propose a simple set of criteria to be used in conjunction with MIQE to reveal the true potential of cell-free microRNAs as biomarkers.
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影响因子：
3.7
作者：
Kirschner MB;Kao SC;Edelman JJ;Armstrong NJ;Vallely MP;van Zandwijk N;Reid G
通讯作者：
Reid G
影响因子：
3.6
作者：
Wong, CH;Song, C;Tan, KC
通讯作者：
Tan, KC
DOI：
10.1073/pnas.0804549105
发表时间：
2008-07-29
影响因子：
11.1
作者：
Mitchell, Patrick S.;Parkin, Rachael K.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh
DOI：
10.1073/pnas.1019055108
发表时间：
2011-03-22
影响因子：
11.1
作者：
Arroyo, Jason D.;Chevillet, John R.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh
影响因子：
3.9
作者：
Mo MH;Chen L;Fu Y;Wang W;Fu SW
通讯作者：
Fu SW