Mycobacterium bovis BCG decreases MHC-II expression in vivo on murine lung macrophages and dendritic cells during aerosol infection.
Mycobacterium bovis BCG decreases MHC-II expression in vivo on murine lung macrophages and dendritic cells during aerosol infection.
复制标题
DOI：
10.1016/j.cellimm.2008.07.002
复制
发表时间：
2009
影响因子：
4.3
通讯作者：
Harding, Clifford V.
中科院分区：
文献类型：
--
作者：
Pecora, Nicole D.;Fulton, Scott A.;Reba, Scott M.;Drage, Michael G.;Simmons, Daimon P.;Urankar-Nagy, Nancy J.;Boom, W. Henry;Harding, Clifford V.
结核分枝杆菌和M.牛BCG感染APC。在体外，分枝杆菌抑制IFN-γ诱导的巨噬细胞MHC-II表达，但分枝杆菌对肺APC的体内作用尚不清楚。为了评估体内感染的APC上的MHC-II表达，用表达GFP的BCG气溶胶感染小鼠。在28天时，通过流式细胞术和CFU数据，~1%的肺APC为GFP+。大多数GFP+细胞是CD 11b高/CD 11 c中肺巨噬细胞（58-68%）或CD 11b高/CD 11 chigh DC（28-31%）。感染小鼠肺APC MHC-II表达高于未感染小鼠。然而，在感染的肺内，对于巨噬细胞和DC，GFP+细胞中的MHC-II表达低于GFP−细胞。纯化的肺巨噬细胞和树突状细胞在体外感染BCG后，MHC-II的表达也受到抑制。因此，肺APC，窝藏分枝杆菌在体内有减少MHC-II表达相对于未感染的APC从同一个肺，可能有助于逃避T细胞反应。
Mycobacterium tuberculosis and M. bovis BCG infect APCs. In vitro, mycobacteria inhibit IFN-gamma-induced MHC-II expression by macrophages, but the effects of mycobacteria on lung APCs in vivo remain unclear. To assess MHC-II expression on APCs infected in vivo, mice were aerosol-infected with GFP-expressing BCG. At 28 d, ~1% of lung APCs were GFP+ by flow cytometry and CFU data. Most GFP+ cells were CD11bhigh/CD11cneg-mid lung macrophages (58–68%) or CD11bhigh/CD11chigh DCs (28–31%). Lung APC MHC-II expression was higher in infected mice than naïve mice. Within infected lungs, however, MHC-II expression was lower in GFP+ cells than GFP− cells for both macrophages and DCs. MHC-II expression was also inhibited on purified lung macrophages and DCs that were infected with BCG in vitro. Thus, lung APCs that harbor mycobacteria in vivo have decreased MHC-II expression relative to uninfected APCs from the same lung, possibly contributing to evasion of T cell responses.
登录
查看更多内容
影响因子：
5.6
作者：
SKLAR, MD;TEREBA, A;WALKER, WS
通讯作者：
WALKER, WS
影响因子：
4.4
作者：
Pecora, Nicole D.;Gehring, Adam J.;Harding, Clifford V.
通讯作者：
Harding, Clifford V.
影响因子：
3.1
作者：
Pai, RK;Pennini, ME;Harding, CV
通讯作者：
Harding, CV
影响因子：
4.4
作者：
Ordway, D;Henao-Tamayo, M;Gonzalez-Juarrero, M
通讯作者：
Gonzalez-Juarrero, M
影响因子：
3.1
作者：
Fulton, SA;Reba, SM;Boom, WH
通讯作者：
Boom, WH