Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells.
Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells.
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DOI：
10.1096/fj.08-123596
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Bennett DC
中科院分区：
文献类型：
--
作者：
Sviderskaya EV;Easty DJ;Lawrence MA;Sánchez DP;Negulyaev YA;Patel RH;Anand P;Korchev YE;Bennett DC
干细胞，即既能自我复制又能分化为功能性细胞类型的细胞，作为治疗和疾病治疗的潜在辅助手段引起了人们的极大兴趣。胚胎神经嵴是多能性的，并产生周围神经系统、黑素细胞和一些结缔组织。神经嵴相关干细胞在出生后的皮肤中已有报道：毛囊中的固定黑色素细胞干细胞，以及来自毛囊和乳头的多能细胞类型，可以产生各种谱系。在这里，我们描述了从新生小鼠表皮中提取的新型多能神经嵴样干细胞，具有不同的效力，分离为3个独立的不朽系。使用其他调节因子，它们可以转化为大量的雪旺前体细胞、色素黑素细胞、软骨细胞或显示电压门控钠通道的功能性感觉神经元。部分神经元表现出丰富的TRPV1和TRPA1活性受体。这种功能神经元以前只能通过外植获得，很难在培养中获得。该系统还用于生成干细胞、黑素细胞和黑素母细胞的比较基因表达数据，充分解释了黑素母细胞中色素的缺乏，并为黑色素瘤中一些明显异位表达的基因（如ephrin受体）提供了理论依据。-Sviderskaya， E. V, Easty， D. J, Lawrence， M. A, Sánchez， D. P, Negulyaev， Y. A., Patel， R. H., Anand， P., Korchev， Y. E., Bennett， D.。从出生后神经嵴样干细胞永生系诱导的功能神经元和黑色素细胞。
Stem cells, that is, cells that can both reproduce themselves and differentiate into functional cell types, attract much interest as potential aids to healing and disease therapy. Embryonic neural crest is pluripotent and generates the peripheral nervous system, melanocytes, and some connective tissues. Neural-crest-related stem cells have been reported previously in postnatal skin: committed melanocytic stem cells in the hair follicle, and pluripotent cell types from the hair follicle and papilla that can produce various sets of lineages. Here we describe novel pluripotent neural crest-like stem cells from neonatal mouse epidermis, with different potencies, isolated as 3 independent immortal lines. Using alternative regulatory factors, they could be converted to large numbers of either Schwann precursor cells, pigmented melanocytes, chondrocytes, or functional sensory neurons showing voltage-gated sodium channels. Some of the neurons displayed abundant active TRPV1 and TRPA1 receptors. Such functional neurons have previously been obtained in culture only with difficulty, by explantation. The system was also used to generate comparative gene expression data for the stem cells, melanocytes, and melanoblasts that sufficiently explain the lack of pigment in melanoblasts and provide a rationale for some genes expressed apparently ectopically in melanomas, such as ephrin receptors.—Sviderskaya, E. V., Easty, D. J., Lawrence, M. A., Sánchez, D. P., Negulyaev, Y. A., Patel, R. H., Anand, P., Korchev, Y. E., Bennett, D. C. Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells.
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影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD
DOI：
10.1073/pnas.97.18.10050
发表时间：
2000-08-29
影响因子：
11.1
作者：
Dunn, KJ;Williams, BO;Pavan, WJ
通讯作者：
Pavan, WJ
影响因子：
4.6
作者：
Joseph, NM;Mukouyama, YS;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
5.3
作者：
Carreira, S;Dexter, TJ;Goding, CR
通讯作者：
Goding, CR
影响因子：
5.3
作者：
Cummins, TR;Dib-Hajj, SD;Waxman, SG
通讯作者：
Waxman, SG