Analysis of the genetic diversity and molecular phylogeography of the endangered wild rose (Rosa rugosa) in China based on chloroplast genes
Analysis of the genetic diversity and molecular phylogeography of the endangered wild rose (Rosa rugosa) in China based on chloroplast genes
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基于叶绿体基因的中国濒危野玫瑰遗传多样性及分子系统发育地理学分析
DOI：
10.1016/j.gecco.2021.e01653
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发表时间：
2021-08
影响因子：
4
通讯作者：
Zheng Yongqi
中科院分区：
文献类型：
--
作者：
Xu Jianjun;Zang Fengqi;Wu Qichao;Wang Yi;Wang Baosheng;Huang Ping;Zang Dekui;Ma Yan;Zheng Yongqi
本研究对中国12个野生玫瑰居群的236个个体的rnL-trnF、rp 120-rps 12和rbcl 3个叶绿体基因序列进行了扩增和测序，以研究其遗传多样性、遗传分化和系谱地理结构，为制定保护策略提供必要的信息。共获得19个单倍型。野生玫瑰居群间的基因流（Nm）为0.7，总体遗传多样性较低（Ht = 0.427 ± 0.0863）。野生蔷薇种群的遗传分化系数Nst = 0.270 > Gst = 0.197（p < 0.01），表明中国野生蔷薇种群具有明显的地理异质性。分子方差分析（AMOVA）结果表明，野蔷薇的遗传变异主要发生在居群内（77.27%），居群间变异较少（22.73%）。此外，野生玫瑰种群表现出很大程度的遗传分化（Fst = 0.2273）。Mantel检验结果表明，地理隔离不是导致野生蔷薇居群间遗传分化的主要因素。错配分析曲线和中性检验表明，野生玫瑰种群最近有扩大的趋势。野生蔷薇可能存在多个避难所，如山东烟台（DYZ）、威海（CSZ）和北部的珲春（JXZ）。造山运动、渤海的形成和人类活动是野生玫瑰在中国分散分布的重要驱动力。
In this study, 3 chloroplast sequences (rnL-trnF, rpl20-rps12, andrbcl) were amplified and sequenced in 236 individuals from 12 wild rose (Rosa rugosa) populations in China to determine their genetic diversity, genetic differentiation and pedigree geographic structure in order to provide information necessary for formulating protection strategies. In total, 19 haplotypes were obtained. The gene flow (Nm) among the wild rose populations was 0.7, and the species exhibited low overall genetic diversity (Ht = 0.427 ± 0.0863). The genetic differentiation coefficients of wild rose were Nst = 0.270 > Gst = 0.197 (p < 0.01), indicating that the wild rose populations in China have obvious phylogeographic structure. Analysis of molecular variance (AMOVA) showed that genetic variation in wild rose occurs mainly within populations (77.27%) rather than between populations (22.73%). Furthermore, the wild rose populations exhibited a large degree of genetic differentiation (Fst = 0.2273). The results of a Mantel test showed that geographical isolation was not the main factor causing the genetic differentiation among the wild rose populations. A mismatch analysis curve and neutrality test indicated that the wild rose populations recently expanded. There may be multiple refuges for wild rose, such as the Shandong Yantai (DYZ) population, the Weihai (CSZ) population in the south, and the Hunchun (JXZ) population in the north. Orogeny, the formation of the Bohai Sea, and human activities are important drivers of the fragmented distribution of wild rose in China.
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DOI：
--
发表时间：
2012
期刊：
--
影响因子：
--
作者：
Sudhir Kumar;A. Drummond;M. Suchard;Dong Xie;A. Rambaut
通讯作者：
Sudhir Kumar;A. Drummond;M. Suchard;Dong Xie;A. Rambaut
影响因子：
2.4
作者：
Y. Zong;P. Sun;Jing Liu;X. Yue;Q. Niu;Yuanwen Teng
通讯作者：
Y. Zong;P. Sun;Jing Liu;X. Yue;Q. Niu;Yuanwen Teng
影响因子：
2.4
作者：
Cubry, Philippe;Gallagher, Evelyn;Kelleher, Colin T.
通讯作者：
Kelleher, Colin T.
影响因子：
2.6
作者：
Greg Ewing-;Allen G. Rodrigo-
通讯作者：
Greg Ewing-;Allen G. Rodrigo-
影响因子：
16.8
作者：
P. Tzedakis;B. Emerson;B. Emerson;G. Hewitt
通讯作者：
P. Tzedakis;B. Emerson;B. Emerson;G. Hewitt