Construction of a New Fusion Anti-caries DNA Vaccine
Construction of a New Fusion Anti-caries DNA Vaccine
复制标题
新型融合抗龋DNA疫苗的构建
DOI：
10.1177/0022034509336727
复制
发表时间：
2009
影响因子：
7.6
通讯作者：
Yuhong Li
中科院分区：
文献类型：
--
作者：
Y. Niu;Jing;M. Fan;Qing;Jihua Guo;R. Jia;Yuhong Li
变形链球菌（MS）通常被认为是龋齿的主要病因。MS有两个重要的毒力因子：细胞表面蛋白PAc和葡萄糖基转移酶（GTFs）。gtf具有两个功能域：n端催化蔗糖结合域（CAT）和c端葡聚糖结合域（GLU）。一种融合抗龋齿DNA疫苗pGJA-P/VAX编码变形链球菌的两个重要抗原结构域PAc和GLU，成功地降低了变形链球菌在非生物动物中引起的龋齿水平。但其对sobrinus感染的保护作用较弱。DNA疫苗是否需要sobrinus的抗原来增强其抑制感染的能力？为了回答这个问题，本研究将S. sobrinus gft -i的催化（cat）片段克隆到pGJA-P/VAX中，该片段显示了S. sobrinus对水不溶性葡聚糖合成的抑制作用，以制备新的抗龋DNA疫苗。
Mutans streptococci (MS) are generally considered to be the principal etiological agent of dental caries. MS have two important virulence factors: cell- surface protein PAc and glucosyltransferases (GTFs). GTFs have two functional domains: an N-terminal catalytic sucrose-binding domain (CAT) and a C-terminal glucan-binding domain (GLU). A fusion anti-caries DNA vaccine, pGJA-P/VAX, encoding two important antigenic domains, PAc and GLU, of S. mutans, was successful in reducing the levels of dental caries caused by S. mutans in gnotobiotic animals. However, its protective effect against S. sobrinus infection proved to be weak. Does the DNA vaccine need an antigen of S. sobrinus to enhance its ability to inhibit infection? To answer this question, in this study, we cloned the catalytic (cat) fragment of S. sobrinus gtf-I, which demonstrated its ability to inhibit water-insoluble glucan synthesis by S. sobrinus, into pGJA-P/VAX to produce a new anti-caries DNA vaccine.
影响因子：
--
作者：
Smith,DJ;Mattos-Graner,RO
通讯作者：
Mattos-Graner,RO
影响因子：
5.5
作者：
Enioutina, EY;Visic, D;Daynes, RA
通讯作者：
Daynes, RA