Consolidated Bioprocessing: Synthetic Biology Routes to Fuels and Fine Chemicals.
Consolidated Bioprocessing: Synthetic Biology Routes to Fuels and Fine Chemicals.
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DOI：
10.3390/microorganisms9051079
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发表时间：
2021-05-18
期刊：
影响因子：
4.5
通讯作者：
Scrutton NS
中科院分区：
文献类型：
--
作者：
Banner A;Toogood HS;Scrutton NS
从新兴生物技术到用于化学品生产的商业“绿色”生物合成路线的漫长道路，在一定程度上取决于微生物有效利用可持续和可再生的废物生物质原料。一种解决方案是应用综合生物处理方法，即微生物将木质纤维素废物转化为先进燃料和其他化学品。由于木质纤维素是一个高度复杂的聚合物网络，酶降解或“糖化”需要一系列的纤维素酶协同作用来释放所含的丰富糖类。并发症产生于需要细胞外定位的纤维素酶，无论它们是游离的还是细胞相关的。这篇综述强调了目前在综合生物处理方法方面的进展，通过这种方法，微生物底盘被设计成以木质纤维素为唯一碳源生长，同时产生商业上有用的化学品。讨论了新出现的细菌宿主生物铸造方法的未来前景，通过应用高通量和迭代的设计-建造-测试-学习方法，有可能克服现有瓶颈的解决方案。这些快速的自动化路径构建基础设施可用于应对将微生物的纤维素分解能力提高到商业可行水平的挑战。
The long road from emerging biotechnologies to commercial “green” biosynthetic routes for chemical production relies in part on efficient microbial use of sustainable and renewable waste biomass feedstocks. One solution is to apply the consolidated bioprocessing approach, whereby microorganisms convert lignocellulose waste into advanced fuels and other chemicals. As lignocellulose is a highly complex network of polymers, enzymatic degradation or “saccharification” requires a range of cellulolytic enzymes acting synergistically to release the abundant sugars contained within. Complications arise from the need for extracellular localisation of cellulolytic enzymes, whether they be free or cell-associated. This review highlights the current progress in the consolidated bioprocessing approach, whereby microbial chassis are engineered to grow on lignocellulose as sole carbon sources whilst generating commercially useful chemicals. Future perspectives in the emerging biofoundry approach with bacterial hosts are discussed, where solutions to existing bottlenecks could potentially be overcome though the application of high throughput and iterative Design-Build-Test-Learn methodologies. These rapid automated pathway building infrastructures could be adapted for addressing the challenges of increasing cellulolytic capabilities of microorganisms to commercially viable levels.
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DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
DOI：
10.1007/978-3-319-43679-1_2
发表时间：
2016-01-01
期刊：
MICROBIAL ENZYMES IN BIOCONVERSIONS OF BIOMASS
影响因子：
--
作者：
Annamalai, Neelamegam;Rajeswari, Mayavan Veeramuthu;Sivakumar, Nallusamy
通讯作者：
Sivakumar, Nallusamy
DOI：
10.1046/j.0014-2956.2001.02475.x
发表时间：
2001-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Ashiuchi, M;Nawa, C;Misono, H
通讯作者：
Misono, H
影响因子：
5.7
作者：
Álvarez C;Reyes-Sosa FM;Díez B
通讯作者：
Díez B
影响因子：
6.4
作者：
Bischof RH;Ramoni J;Seiboth B
通讯作者：
Seiboth B