Identifying (non-)coding RNAs and small peptides: challenges and opportunities.
Identifying (non-)coding RNAs and small peptides: challenges and opportunities.
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DOI：
10.1002/bies.201400103
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发表时间：
2015-01
期刊：
影响因子：
4
通讯作者：
Schier, Alexander F.
中科院分区：
文献类型：
--
作者：
Pauli, Andrea;Valen, Eivind;Schier, Alexander F.
在过去的十年中，高通量研究已经确定了许多新的转录本。虽然它们的存在是无可争议的，但它们的编码潜力和功能仍然存在争议。最近由核糖体分析指导的计算方法表明，翻译比预期的要普遍得多，并且发生在许多以前被认为是非编码的转录本上。这些新发现的翻译转录物中有一些编码了在之前的筛选中缺失的短功能蛋白质。其他转录本被翻译，但它可能是翻译的过程，而不是产生肽的功能。在这里，我们回顾了斑马鱼的注释研究，讨论了将rna放置在连续体上的挑战，从功能性蛋白质编码mrna到潜在的非功能性肽产生rna再到非编码rna。正如新的信号肽Apela/ELABELA/Toddler的发现所强调的那样，准确的注释可以为识别以前未表征的转录本的功能提供令人兴奋的机会。
Over the past decade, high-throughput studies have identified many novel transcripts. While their existence is undisputed, their coding potential and functionality have remained controversial. Recent computational approaches guided by ribosome profiling have indicated that translation is far more pervasive than anticipated and takes place on many transcripts previously assumed to be non-coding. Some of these newly discovered translated transcripts encode short, functional proteins that had been missed in prior screens. Other transcripts are translated, but it might be the process of translation rather than the resulting peptides that serve a function. Here, we review annotation studies in zebrafish to discuss the challenges of placing RNAs onto the continuum that ranges from functional protein-encoding mRNAs to potentially non-functional peptide-producing RNAs to non-coding RNAs. As highlighted by the discovery of the novel signaling peptide Apela/ELABELA/Toddler, accurate annotations can give rise to exciting opportunities to identify the functions of previously uncharacterized transcripts.
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