Fusion-related host proteins are actively regulated by NA during influenza infection as revealed by quantitative proteomics analysis.
Fusion-related host proteins are actively regulated by NA during influenza infection as revealed by quantitative proteomics analysis.
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定量蛋白质组学分析表明，在流感感染期间，融合相关宿主蛋白受到 NA 的主动调节。
DOI：
10.1371/journal.pone.0105947
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Sui Z;Wen B;Gao Z;Chen Q
以A/PR/8/34（PR 8）为背景，构建了3株具有不同神经氨酸酶（NA）的重组A型流感病毒，分别命名为rPR 8-H5 N1 NA、rPR 8-H9 N2 NA和rPR 8-H1N1 NA，分别来源于H5 N1、H9 N2和H1N1（猪）病毒。我们进行了定量蛋白质组学分析，以研究差异蛋白质表达的Madin-Darby犬肾（MDCK）细胞感染重组和野生型流感病毒，以确定是否NA替换会改变宿主细胞基因表达。利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-TOF MS）和双向凝胶电泳（2-DE），我们鉴定了12个上调和49个下调的蛋白质点，包括细胞骨架蛋白、分子生物合成蛋白、泛素-蛋白酶体途径蛋白和热休克蛋白。在感染细胞中观察到分子生物合成蛋白质的最显著变化。我们发现rPR 8-H5 N1 NA或rPR 8-H9 N2 NA病毒感染的细胞中的差异表达蛋白点比野生型病毒感染的细胞中的差异表达蛋白点多。这些蛋白质中的许多被假定参与细胞-细胞融合，但完整的机制仍有待探索。同时，我们的数据表明，野生型病毒比重组病毒具有进化优势。
Three recombinant influenza A viruses with different neuraminidases (NAs) in the background of A/PR/8/34 (PR8), named rPR8-H5N1NA, rPR8-H9N2NA, and rPR8-H1N1NA, derived from H5N1, H9N2, H1N1 (swine) viruses, respectively, were constructed. We performed a quantitative proteomics analysis to investigate differential protein expression in Madin-Darby canine kidney (MDCK) cells infected with recombinant and wild-type influenza viruses to determine whether NA replacement would alter host cell gene expression. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-TOF MS) and two-dimensional gel electrophoresis (2-DE), we identified 12 up-regulated and 49 down-regulated protein spots, including cytoskeletal proteins, molecular biosynthesis proteins, ubiquitin-proteasome pathway proteins, and heat shock proteins. The most significant changes in infected cells were observed for molecular biosynthesis proteins. We found more differentially expressed protein spots in cells infected with rPR8-H5N1NA or rPR8-H9N2NA viruses than cells infected with wild-type virus. Many of those proteins are postulated to be involved in cell-cell fusion, but the full mechanism remains to be explored. Meanwhile, our data demonstrate that the wild-type virus has evolutionary advantages over recombinant viruses.
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DOI：
10.1042/bj20100985
发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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