Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation.
Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation.
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DOI：
10.1016/j.cub.2022.08.014
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发表时间：
2022-10-10
期刊：
影响因子：
9.2
通讯作者：
Karatekin, Erdem
中科院分区：
文献类型：
--
作者：
Landajuela, Ane;Braun, Martha;Martinez-Calvo, Alejandro;Rodrigues, Christopher D. A.;Perez, Carolina Gomis;Doan, Thierry;Rudner, David Z.;Wingreen, Ned S.;Karatekin, Erdem
细菌的细胞分裂和内孢子的形成都需要膜裂变。在枯草芽孢杆菌中，产孢开始于不对称分裂，产生一个大的母细胞和一个小的前孢子，前孢子只包含其基因组的四分之一。当母细胞膜吞没前孢子时，DNA转位酶将染色体的其余部分泵入小的前孢子室，使其膨胀。当吞噬膜发生裂变时，前孢子被释放到母细胞的细胞质中。枯草芽孢杆菌蛋白FisB在产孢过程中催化膜裂变，但分子基础尚不清楚。在这里，我们表明前孢子膨胀和FisB积累都是有效膜裂变所必需的。前孢子膨胀导致吞噬膜的膜张力高于母细胞膜，导致膜通过连接两个膜室的颈部流动。因此，母细胞提供前孢子周围膜生长所需的一些膜。在膜颈处的FisB寡聚化通过阻碍膜流动减缓了膜张力的平衡。这导致吞噬膜的张力进一步增加，通过裂解促进其裂变。总的来说，我们的数据表明，在能量有限的条件下，dna易位在激活fish介导的膜裂变方面具有以前未被认识到的第二功能。Landajuela等人的研究表明，枯草芽孢杆菌孢子内形成过程中膜颈的裂变是由于膜颈一侧膜张力增加与其中聚集了一簇fish b蛋白之间的相互作用，阻碍了膜通量和张力平衡。
Bacteria require membrane fission for both cell division and endospore formation. In Bacillus subtilis, sporulation initiates with an asymmetric division that generates a large mother cell and a smaller forespore that contains only a quarter of its genome. As the mother cell membranes engulf the forespore, a DNA translocase pumps the rest of the chromosome into the small forespore compartment, inflating it due to increased turgor. When the engulfing membrane undergoes fission, the forespore is released into the mother cell cytoplasm. The B. subtilis protein FisB catalyzes membrane fission during sporulation, but the molecular basis is unclear. Here we show that forespore inflation and FisB accumulation are both required for efficient membrane fission. Forespore inflation leads to higher membrane tension in the engulfment membrane than in the mother cell membrane, causing membrane to flow through the neck connecting the two membrane compartments. Thus, the mother cell supplies some of the membrane required for the growth of the membranes surrounding the forespore. Oligomerization of FisB at the membrane neck slows equilibration of membrane tension by impeding membrane flow. This leads to a further increase in the tension of the engulfment membrane, promoting its fission through lysis. Collectively our data indicate that DNA-translocation has a previously unappreciated second function in energizing FisB-mediated membrane fission under energy-limited conditions. Landajuela et al. show that fission of a membrane neck during endospore formation in Bacillus subtilis results from an interplay between increasing membrane tension on one side of the neck and accumulation of a cluster of FisB proteins inside it, impeding membrane flux and tension equilibration.
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