Engineering three-dimensional stem cell morphogenesis for the development of tissue models and scalable regenerative therapeutics.
Engineering three-dimensional stem cell morphogenesis for the development of tissue models and scalable regenerative therapeutics.
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工程三维干细胞形态发生，用于开发组织模型和可扩展的再生治疗剂。
DOI：
10.1007/s10439-013-0953-9
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发表时间：
2014-02
影响因子：
3.8
通讯作者：
McDevitt, Todd C.
中科院分区：
文献类型：
--
作者：
Kinney, Melissa A.;Hookway, Tracy A.;Wang, Yun;McDevitt, Todd C.
关键词：
干细胞的物理化学微环境调节着自我更新和分化之间的微妙平衡。干细胞的三维组装促进了细胞相互作用，促进了形态发生，类似于伴随胚胎发生的多细胞异型组织组织。因此，干细胞作为多细胞聚集体的扩增和分化为研究时空形态发生和组织模式背后的生物和工程原理提供了一个可控的平台。此外，三维干细胞培养适用于翻译筛选应用和治疗，这强调了可扩展的悬浮培养在实验室和临床规模上的广泛应用。在这篇综述中，我们在基本生物物理原理的背景下讨论了干细胞的形态发生，包括粘附、力学和分子运输的三维调节，并强调了利用干细胞球体进行组织建模、生物加工和再生治疗的机会。
The physiochemical stem cell microenvironment regulates the delicate balance between self-renewal and differentiation. The three-dimensional assembly of stem cells facilitates cellular interactions that promote morphogenesis, analogous to the multicellular, heterotypic tissue organization that accompanies embryogenesis. Therefore, expansion and differentiation of stem cells as multicellular aggregates provides a controlled platform for studying the biological and engineering principles underlying spatiotemporal morphogenesis and tissue patterning. Moreover, three-dimensional stem cell cultures are amenable to translational screening applications and therapies, which underscores the broad utility of scalable suspension cultures across laboratory and clinical scales. In this review, we discuss stem cell morphogenesis in the context of fundamental biophysical principles, including the three-dimensional modulation of adhesions, mechanics, and molecular transport and highlight the opportunities to employ stem cell spheroids for tissue modeling, bioprocessing, and regenerative therapies.
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影响因子：
3.7
作者：
Bauer M;Kang L;Qiu Y;Wu J;Peng M;Chen HH;Camci-Unal G;Bayomy AF;Sosnovik DE;Khademhosseini A;Liao R
通讯作者：
Liao R
影响因子：
14
作者：
Carpenedo, Richard L.;Bratt-Leal, Andrs M.;Marklein, Ross A.;Seaman, Scott A.;Bowen, Nathan J.;McDonald, John F.;McDevitt, Todd C.
通讯作者：
McDevitt, Todd C.
影响因子：
3.8
作者：
Bauwens, C;Yin, T;Zandstra, PW
通讯作者：
Zandstra, PW
影响因子：
4.9
作者：
Carpenedo, Richard L.;Seaman, Scott A.;McDevitt, Todd C.
通讯作者：
McDevitt, Todd C.
影响因子：
14
作者：
Choi, Jeein;Kim, Sohyeun;Kang, Sookyung
通讯作者：
Kang, Sookyung