The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells.
The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells.
复制标题
催化铁在石棉诱导 C3H10T1/2 细胞脂质过氧化和 DNA 链断裂中的作用。
作者：
C. Turver;RC Brown
已经研究了催化铁在青石棉体外活性中的作用。将 C3H10T1/2 细胞暴露于 UICC 青石棉标准参考样品或非纤维（研磨）衍生物会导致硫代巴比妥酸反应物质增加。用铁螯合剂去铁胺预处理可抑制该催化活性。通过基于 DNA 链断裂产生的测定来测量这种活性对细胞 DNA 的影响。在用研磨青石棉和 UICC 青石棉处理的培养物中检测到 DNA 链断裂水平增加。石棉中加入去铁敏可抑制 DNA 链断裂。结论是粉尘中存在的催化铁能够破坏脂质和 DNA，这可能是石棉致病性的重要机制。
The involvement of catalytic iron in the vitro activities of crocidolite asbestos has been investigated. Exposure of C3H10T1/2 cells to either the UICC crocidolite standard reference sample or a non fibrous (milled) derivative resulted in an increase of thiobarbituric acid reactive substances. This catalytic activity was inhibited by pretreatment with the iron chelator desferrioxamine. The effect of this activity on cellular DNA was measured in an assay based on the production of DNA-strand breaks. Increased levels of DNA-strand breaks were detected in cultures treated with both the milled and UICC crocidolite. Inclusion of desferrioxamine with the asbestos inhibited DNA-strand breakage. It is concluded the catalytic iron present on the dust is capable of damaging both lipid and DNA and that this could be an important mechanism in asbestos pathogenicity.
石棉引起的过氧化氢分解。
影响因子：
8.3
作者：
Eberhardt,MK;Román-Franco,AA;Quiles,MR
通讯作者：
Quiles,MR
石棉纤维对多形核白细胞功能的体外影响。
DOI：
10.1159/000233061
发表时间：
1982
期刊：
International archives of allergy and applied immunology
影响因子：
--
作者：
Doll,NJ;Stankus,RP;Goldbach,S;Salvaggio,JE
通讯作者：
Salvaggio,JE