Protein kinase A controls yeast growth in visible light.
Protein kinase A controls yeast growth in visible light.
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DOI：
10.1186/s12915-020-00867-4
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发表时间：
2020-11-16
期刊：
影响因子：
5.4
通讯作者：
Blomberg A
中科院分区：
文献类型：
--
作者：
Molin M;Logg K;Bodvard K;Peeters K;Forsmark A;Roger F;Jörhov A;Mishra N;Billod JM;Amir S;Andersson M;Eriksson LA;Warringer J;Käll M;Blomberg A
各种各样的光合和非光合物种感知和响应光，已经开发出保护机制，以适应对DNA和蛋白质的破坏作用。虽然紫外线引起的损伤的生物学已经得到了很好的研究，但细胞对可见光（400-700 nm）的应激反应仍然知之甚少，尽管这是许多生物体生命周期的常规部分。在这里，我们开发了一种高通量的方法来测量生长在可见光胁迫下，并使用它来筛选酵母基因缺失收集的光敏感性。我们发现基因参与HOG途径信号传导，RNA聚合酶II转录，翻译，翻译延伸因子eEF 2的二苯二甲酰胺修饰，以及光抗性所需的氧化应激反应。减少核定位的转录因子Msn 2和较低的糖原积累表明较高的蛋白激酶A（cAMP依赖性蛋白激酶，PKA）的活性在许多光敏基因缺失株。因此，我们使用了一个异位荧光PKA报告和突变体与组成性改变PKA活性，以表明抑制PKA是必不可少的耐可见光。我们的结论是，酵母光生物学是多方面的，蛋白激酶A在可见光照射下细胞生长的能力中起着关键作用。我们认为，可见光对许多非光合生物的生物学和进化产生影响，并对在实验室中如何研究生物体具有实际意义，无论是否有照明。
A wide variety of photosynthetic and non-photosynthetic species sense and respond to light, having developed protective mechanisms to adapt to damaging effects on DNA and proteins. While the biology of UV light-induced damage has been well studied, cellular responses to stress from visible light (400–700 nm) remain poorly understood despite being a regular part of the life cycle of many organisms. Here, we developed a high-throughput method for measuring growth under visible light stress and used it to screen for light sensitivity in the yeast gene deletion collection. We found genes involved in HOG pathway signaling, RNA polymerase II transcription, translation, diphthamide modifications of the translational elongation factor eEF2, and the oxidative stress response to be required for light resistance. Reduced nuclear localization of the transcription factor Msn2 and lower glycogen accumulation indicated higher protein kinase A (cAMP-dependent protein kinase, PKA) activity in many light-sensitive gene deletion strains. We therefore used an ectopic fluorescent PKA reporter and mutants with constitutively altered PKA activity to show that repression of PKA is essential for resistance to visible light. We conclude that yeast photobiology is multifaceted and that protein kinase A plays a key role in the ability of cells to grow upon visible light exposure. We propose that visible light impacts on the biology and evolution of many non-photosynthetic organisms and have practical implications for how organisms are studied in the laboratory, with or without illumination.
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影响因子：
11.4
作者：
Chen, Chen-Hui;Ringelberg, Carol S.;Loros, Jennifer J.
通讯作者：
Loros, Jennifer J.
DOI：
10.1016/j.bbamcr.2010.12.004
发表时间：
2011-02-01
影响因子：
5.1
作者：
Bodvard, Kristofer;Wrangborg, David;Kall, Mikael
通讯作者：
Kall, Mikael
影响因子：
--
作者：
Depry, Charlene;Allen, Michael D.;Zhang, Jin
通讯作者：
Zhang, Jin
在基因组量表上对酵母中适应性和遗传相互作用的定量分析。
影响因子：
48
作者：
Baryshnikova, Anastasia;Costanzo, Michael;Kim, Yungil;Ding, Huiming;Koh, Judice;Toufighi, Kiana;Youn, Ji-Young;Ou, Jiongwen;San Luis, Bryan-Joseph;Bandyopadhyay, Sunayan;Hibbs, Matthew;Hess, David;Gingras, Anne-Claude;Bader, Gary D.;Troyanskaya, Olga G.;Brown, Grant W.;Andrews, Brenda;Boone, Charles;Myers, Chad L.
通讯作者：
Myers, Chad L.
影响因子：
4.4
作者：
BECKE, AD
通讯作者：
BECKE, AD