Identification of STAU1 as a regulator of HBV replication by TurboID-based proximity labeling.
Identification of STAU1 as a regulator of HBV replication by TurboID-based proximity labeling.
复制标题
通过基于 TurboID 的邻近标记鉴定 STAU1 作为 HBV 复制的调节因子
DOI：
10.1016/j.isci.2022.104416
复制
发表时间：
2022-06-17
期刊：
影响因子：
5.8
通讯作者：
Hu, Jie-Li
中科院分区：
文献类型：
--
作者：
Wei, Xia-Fei;Fan, Shu-Ying;Wang, Yu-Wei;Li, Shan;Long, Shao-Yuan;Gan, Chun-Yang;Li, Jie;Sun, Yu-Xue;Guo, Lin;Wang, Pei-Yun;Yang, Xue;Wang, Jin-Lan;Cui, Jing;Zhang, Wen-Lu;Huang, Ai-Long;Hu, Jie-Li
乙肝病毒核心启动子(CP)通过控制前基因组RNA(PgRNA)的转录，对乙肝病毒的复制起着至关重要的作用。调节CP活性的寄主因子可以通过不同的方法来鉴定。基于生物素的邻近标记是一种能够捕捉弱相互作用或动态相互作用的强大方法，但尚未被用于定位与CP相互作用的蛋白质。在这里，我们建立了一种基于新进化的混杂酶TurboID的策略，用于询问调节HBVCP活性的宿主因素。利用这一策略，我们确定STAU1是参与调节HBVCP的一个重要因子。在机制上，STAU1与TARDBP介导的CP间接结合，并将sagA转录共激活复合体招募到CP以上调其活性。此外，STAU1与HBx结合，通过以泛素不依赖的方式稳定HBx，从而提高HBx的水平。STAU1通过TARDBP间接与核心启动子结合并招募SAGA复合体STAU1与HBx结合并增加其稳定性分子生物学；病毒学
The core promoter (CP) of hepatitis B virus (HBV) is critical for HBV replication by controlling the transcription of pregenomic RNA (pgRNA). Host factors regulating the activity of the CP can be identified by different methods. Biotin-based proximity labeling, a powerful method with the capability to capture weak or dynamic interactions, has not yet been used to map proteins interacting with the CP. Here, we established a strategy, based on the newly evolved promiscuous enzyme TurboID, for interrogating host factors regulating the activity of HBV CP. Using this strategy, we identified STAU1 as an important factor involved in the regulation of HBV CP. Mechanistically, STAU1 indirectly binds to CP mediated by TARDBP, and recruits the SAGA transcription coactivator complex to the CP to upregulate its activity. Moreover, STAU1 binds to HBx and enhances the level of HBx by stabilizing it in a ubiquitin-independent manner. HBV core promoter binding factors were interrogated by a proximity labeling method STAU1 enhances HBV transcription and replication STAU1 indirectly binds to core promoter via TARDBP and recruits SAGA complex STAU1 binds to HBx and increases its stability Molecular biology; Virology
登录
查看更多内容
影响因子：
48
作者：
Gao XD;Tu LC;Mir A;Rodriguez T;Ding Y;Leszyk J;Dekker J;Shaffer SA;Zhu LJ;Wolfe SA;Sontheimer EJ
通讯作者：
Sontheimer EJ
影响因子：
5.3
作者：
Hu J;Cheng J;Tang L;Hu Z;Luo Y;Li Y;Zhou T;Chang J;Guo JT
通讯作者：
Guo JT
影响因子：
5.4
作者：
de Lucas, Susana;Peredo, Joan;Ortin, Juan
通讯作者：
Ortin, Juan
影响因子：
7.7
作者：
Bozal-Basterra, Laura;Gonzalez-Santamarta, Maria;Barrio, Rosa
通讯作者：
Barrio, Rosa
影响因子：
10.5
作者：
Bonnet J;Wang CY;Baptista T;Vincent SD;Hsiao WC;Stierle M;Kao CF;Tora L;Devys D
通讯作者：
Devys D