Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection.
Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection.
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草鱼呼肠孤病毒 (GCRV) 感染后草鱼体内整体和补体基因特异性 DNA 甲基化
DOI：
10.3390/ijms19041110
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发表时间：
2018-04-07
影响因子：
5.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Xiong L;He L;Luo L;Li Y;Liao L;Huang R;Zhu Z;Wang Y
草鱼呼肠孤病毒（Grasscarpreovirus，GCRV）给草鱼养殖业造成了巨大的经济损失，但其致病机制尚不清楚。本研究旨在研究草鱼感染GCRV后，其DNA甲基化水平的变化，以揭示GCRV感染草鱼的机制。GCRV感染后，DNA甲基化水平升高。在GCRV感染后，DNA甲基化相关酶的表达水平发生了显著变化，包括DNA甲基转移酶（DNMT）、10 - 11易位蛋白（THEX）和甘氨酸N-甲基转移酶（GNMT）。为了研究基因表达水平与DNA甲基化水平之间的关系，选择了两个代表性的补体基因，补体成分3（C3）和激肽原-1（KNG 1）进行进一步分析。GCRV感染后5、7 d，两个基因的mRNA表达水平均显著升高，而5′端DNA甲基化水平均显著降低。此外，基因表达水平和DNA甲基化水平之间检测到负相关。因此，目前的数据揭示了GCRV感染后的全局和补体基因特异性DNA甲基化谱。我们的研究将为理解GCRV感染的机制提供新的见解。
Grass carp reovirus (GCRV) causes huge economic loss to the grass carp cultivation industry but the mechanism remains largely unknown. In this study, we investigated the global and complement gene-specific DNA methylation in grass carp after GCRV infection aimed to uncover the mechanism underlying GCRV infection. The global DNA methylation level was increased after GCRV infection. Expression levels of enzymes involved in DNA methylation including DNA methyltransferase (DNMT), ten-eleven translocation proteins (TETs), and glycine N-methyltransferase (GNMT) were significantly altered after GCRV infection. In order to investigate the relationship between the gene expression level and DNA methylation level, two representative complement genes, complement component 3 (C3) and kininogen-1 (KNG1), were selected for further analysis. mRNA expression levels of the two genes were significantly increased at 5 and 7 days after GCRV infection, whereas the DNA methylation level at the 5′ flanking regions of the two genes were down-regulated at the same time-points. Moreover, a negative correlation was detected between gene expression levels and DNA methylation levels of the two genes. Therefore, the current data revealed a global and complement gene-specific DNA methylation profile after GCRV infection. Our study would provide new insights into understanding the mechanism underlying GCRV infection.
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