Ribonucleotide and ribonucleoside determination by ambient pressure ion mobility spectrometry.
Ribonucleotide and ribonucleoside determination by ambient pressure ion mobility spectrometry.
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DOI：
10.1016/j.aca.2009.10.058
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发表时间：
2010-01-18
影响因子：
6.2
通讯作者：
Hill, Herbert H., Jr.
中科院分区：
文献类型：
--
作者：
Kanu, Abu B.;Hampikian, Greg;Brandt, Simon D.;Hill, Herbert H., Jr.
采用常压电喷雾离子迁移谱法（ESI-IMS）测定了12种核糖核苷酸和4种核糖核苷的还原迁移率、分辨能力和检出限。使用本研究中使用的仪器，可以从混合物中分离出其中的一些化合物。此外，核糖核苷酸和核糖核苷的检出限为15 ~ 300皮摩尔，分辨能力为41 ~ 56皮摩尔，表明常压ESI-IMS可用于快速、灵敏的分离和检测。这个简短的报告表明，可以使用IMS在不到一秒的时间内分离核苷酸和核苷。该应用程序在基因组测序中分离DNA片段的核苷酸分析领域以及在犯罪现场鉴定血迹和亲子鉴定中用于法医DNA分型检查方面具有很大的前景。
Reduced mobilities, resolving powers and detection limits for 12 ribonucleotides and 4 ribonucleosides were measured by ambient pressure electrospray ionization ion mobility spectrometry (ESI-IMS). With the instrument used in this study it was possible to separate some of these compounds within the mixtures. In addition, the detection limits reported for the ribonucleotides and ribonucleosides ranged from 15 to 300 picomoles whereas resolving power ranged from 41 to 56 suggesting that ambient pressure ESI-IMS may be used for their rapid and sensitive separation and detection. This short report demonstrates that it was possible to use IMS for the separation of nucleotides and nucleosides in less than one second. The application holds great promise for nucleotide analysis in the area of separating DNA fragments in genome sequencing and also for forensics DNA typing examinations used for the identification of blood stains in crime scenes and paternity testing.
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影响因子：
2.9
作者：
Liu, CC;Huang, JS;Dovichi, NJ
通讯作者：
Dovichi, NJ
影响因子：
4.8
作者：
Cok, SJ;Acton, SJ;Morrison, AR
通讯作者：
Morrison, AR
影响因子：
7.4
作者：
Fundador, Erwin V.;Choudhary, Dharamainder;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
7.4
作者：
ROKUSHIKA, S;HATANO, H;HILL, HH
通讯作者：
HILL, HH
DOI：
10.1016/s1044-0305(02)00866-8
发表时间：
2003-02-01
影响因子：
3.2
作者：
Gidden, J;Bowers, MT
通讯作者：
Bowers, MT