Cyclosporine toxicity on cultured rat microvascular endothelial cells.
Cyclosporine toxicity on cultured rat microvascular endothelial cells.
复制标题
环孢素对培养的大鼠微血管内皮细胞的毒性。
作者：
David C.W. Lau;Kar;Wei
本研究旨在探讨环孢素肾毒性和高血压的机制。将培养的大鼠微血管内皮细胞暴露于下列浓度之一的环孢素：10、50、250和1000 ng/ml的培养液中，最多6天。环孢素以剂量依赖的方式抑制内皮细胞复制；在较高浓度(250和1000 ng/ml)时，细胞复制在治疗后4天和6天减少多达70%到90%，没有证据表明细胞死亡增加。经药物处理的内皮细胞出现胞浆小泡形成、核仁改变等异常形态改变。加入环孢素后，内皮细胞释放前列环素的量增加约3倍(P<0.01)。在环孢菌素存在或不存在的情况下，吲哚美辛显著减少内皮细胞释放前列环素(P<0.01)。我们的数据表明，环孢素诱导的肾毒性可能至少部分是通过环孢素抑制微血管内皮细胞对损伤的再生反应，以及由此导致的前列环素产生的改变来实现的。
The present study was undertaken to study the mechanism of cyclosporine-induced nephrotoxicity and hypertension. Cultured rat microvascular endothelial cells were exposed to cyclosporine for up to six days at one of the following concentrations: 10, 50, 250, and 1000 ng/ml of culture medium. Cyclosporine inhibited endothelial cell replication in a dose-dependent manner; at higher concentrations (250 and 1000 ng/ml), cell replication decreased by as much as 70 to 90% of controls at four and six days post-treatment, with no evidence of increased cell death. Drug-treated endothelial cells revealed abnormal morphological changes such as formation of cytoplasmic vesicles and nucleolar changes. Prostacyclin release by endothelial cells was increased by about threefold with the addition of cyclosporine (P less than 0.01). Indomethacin significantly decreased prostacyclin release by endothelial cells in the presence or absence of cyclosporine (P less than 0.01). Our data suggest that cyclosporine-induced nephrotoxicity may be mediated, at least partly, by the inhibitory influence of cyclosporine on the regenerative response of microvascular endothelial cells to injury, and the resultant alterations in prostacyclin production by these cells.
人血浆脂蛋白对培养内皮细胞产生前列环素的影响。
影响因子：
6.5
作者：
Spector,AA;Scanu,AM;Kaduce,TL;Figard,PH;Fless,GM;Czervionke,RL
通讯作者：
Czervionke,RL
使用对苯二胺封固缓冲液增强特异性免疫荧光结果。
DOI：
10.1111/1523-1747.ep12508101
发表时间：
1982
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Huff,JC;Weston,WL;Wanda,KD
通讯作者：
Wanda,KD
影响因子：
158.5
作者：
MYERS, BD;ROSS, J;PERLROTH, M
通讯作者：
PERLROTH, M