Enhancement of specific immunofluorescent findings with use of a para-phenylenediamine mounting buffer.
Enhancement of specific immunofluorescent findings with use of a para-phenylenediamine mounting buffer.
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使用对苯二胺封固缓冲液增强特异性免疫荧光结果。
DOI：
10.1111/1523-1747.ep12508101
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Wanda,KD
中科院分区：
文献类型：
--
作者：
Huff,JC;Weston,WL;Wanda,KD
最近描述的免疫荧光封片缓冲液含有对苯二胺防止褪色的特定染色在皮肤切片在显微镜检查，并允许更好地欣赏Illorphological细节。在高功率和强烈照明下检查载玻片，以及改进的photoxn。在免疫荧光显微镜下检查用荧光素标记的抗体染色的皮肤切片时，特异性染色会褪色[1，2]。这种褪色是特别突出的，当标本检查与高功率物镜与显微镜配备了epillUlllium系统和汞光源。由于这种现象，很难或不可能在高功率下仔细检查细微的免疫荧光结果并对这些结果进行摄影。约翰逊和Nogueira-Araujo最近描述了一种在抗核抗体试验中检查细胞时减少阳性免疫荧光Imdings褪色的简单方法[3]。该方法涉及在封固缓冲液中使用对苯二胺（PPD）。我们已经使用TLIS缓冲液通过免疫荧光检测皮肤标本，发现特异性染色的褪色是最小的。该试剂允许对免疫荧光结果进行高倍检查和照相，这在以前是不可能的。该试剂的使用大大方便了我们的免疫荧光检查，我们希望引起其他人的注意。
A recently described immunofluorescence mounting buffer containing para-phenylenediamine prevents fading of specific staining in skin sections during microscopic examination, and allows better appreciation of Illorphological detail. Examination of slides at high powers with intense illumination, as well as improved photoxn. icrographs, are possible with this reagent.Examination of skin sections stained with fluorescein-tagged antibodies under the immunofluorescence microscope is accompanied by fading of specific staining [1, 2]. This fading is particularly prominent when specimens are examined with high-power objectives with microscopes equipped with epiillUlllination systems and mercury light sources. As a result of this phenomenon, careful examination of subtle immunofluorescence findings at high powers and photography of these findings are difficult or impossible. Johnson and Nogueira-Araujo have recently described a simple m ethod of reducing fading of positive immunofluorescent Imdings in the examination of cells in an antinuclear antibody test [3]. This method involves the use of para-phenylenediamine (PPD) in the mounting buffer. We have used tllis buffer in the examination of skin specimens by du'ect immunofluorescence and have found fading of specific staining to be minimal. This reagent has allowed high power examination and photography of immunofluorescence findings that were not previously possible. Use of this reagent has so facilitated our immunofluorescence examinations, that we wish to bring this method to the attention of others.
人类坏死性血管炎：皮肤病变和正常皮肤血管壁中的免疫球蛋白和补体。
DOI：
10.1111/1523-1747.ep12512411
发表时间：
1975
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
W. Sams;H. Claman;Peter F. Kohler;Rawle M. McIntosh;Peter. Small;M.F. Mass
通讯作者：
M.F. Mass