Oral lichen planus and malignant transformation: The role of p16, Ki-67, Bub-3 and SOX4 in assessing precancerous potential.
Oral lichen planus and malignant transformation: The role of p16, Ki-67, Bub-3 and SOX4 in assessing precancerous potential.
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DOI：
10.3892/etm.2018.5971
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发表时间：
2018-05
影响因子：
2.7
通讯作者：
Amorim RFB
中科院分区：
文献类型：
--
作者：
Rosa EA;Hurtado-Puerto AM;Falcão DP;Brietzke AP;De Almeida Prado Franceschi LE;Cavalcanti Neto FF;Tiziane V;Carneiro FP;Kogawa EM;Moreno H;Amorim RFB
口腔扁平苔藓（OLP）病变与恶变风险的关联仍然是一个有争议的话题，并且具有临床重要性。因此，本研究评估了 p16、Ki-67、不受苯并咪唑抑制的出芽 3 (Bub-3) 和性别决定区 Y 相关高迁移率族蛋白 4 (SOX4) 的表达水平，以及它们作为 OLP 癌前生物标志物的作用。进行了一项回顾性研究，其中使用了 OLP、口腔发育不良 (OD)、皮肤扁平苔藓 (CLP) 和口腔纤维增生 (OFH) 的组织块 (n=120)。计算每组中p16、BUB3、Ki-67和SOX4表达的阳性指数(PI)。 p16的PI分别为：OLP为20.65%、OD为7.85%、CLP为86.59%、OFH为11.8%，各组间差异有统计学意义（P<0.001）。 Ki-67的PI分别为OLP为11.6%、OD为14.4%、CLP为8.24%、OFH为5.5%，各组之间观察到统计学显着差异（P<0.001）。值得注意的是，BUB3的表达水平在各组之间没有统计学差异。 SOX4 的最高表达水平在 CLP 中被发现（与 OLP/CLP 相比，P<0.001；与 CLP/OD 相比，P=0.001）。 p16和Ki-67表达水平的测定表明，特定的OLP病变可能具有中等恶性潜力，应仔细随访。 CLP 中强烈的 SOX4 染色表明上皮细胞与口腔粘膜细胞的增殖模式不同。这些发现表明SOX4表达也可能与OLP和CLP的不同临床病程有关。
The association of oral lichen planus (OLP) lesions with malignant transformation risk has remained a controversial topic and is of clinical importance. Therefore, the present study evaluated the expression levels of p16, Ki-67, budding uninhibited by benzimidazoles 3 (Bub-3) and sex-determining region Y-related high mobility group box 4 (SOX4), and their roles as precancerous biomarkers in OLP. A retrospective study was performed, in which tissue blocks of OLP, oral dysplasia (OD), cutaneous lichen planus (CLP) and oral fibrous hyperplasia (OFH) were used (n=120). A positivity index (PI) for p16, BUB3, Ki-67 and SOX4 expression was calculated in each group. The PI for p16 was 20.65% for OLP, 7.85% for OD, 86.59% for CLP and 11.8% for OFH, and the difference between these groups was statistically significant (P<0.001). PIs of Ki-67 were indicated as 11.6% for OLP, 14.4% for OD, 8.24% for CLP and 5.5% for OFH, and a statistically significant difference was observed between the groups (P<0.001). Notably, the expression levels of BUB3 were not statistically different among groups. The highest expression levels of SOX4 were identified in CLP (P<0.001 vs. OLP/CLP; P=0,001 vs. CLP/OD). The determined expression levels of p16 and Ki-67 suggest that specific OLP lesions may have an intermediate malignant potential and should be carefully followed up. The intense SOX4 staining in CLP indicated a different proliferation pattern of epithelium compared with oral mucosa cells. These findings suggest that SOX4 expression may also be associated with the different clinical courses of OLP and CLP.
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DOI：
10.1590/1678-775720150058
发表时间：
2015-07
期刊：
Journal of applied oral science : revista FOB
影响因子：
--
作者：
Rosa EA;Lia EN;Macedo SB;Amorim RF
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Amorim RF
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DOI：
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发表时间：
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影响因子：
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2013-04-29
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The Journal of cell biology
影响因子：
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