Uroporphyrin accumulation in the bone marrow of rats exposed to lead.
Uroporphyrin accumulation in the bone marrow of rats exposed to lead.
复制标题
暴露于铅的大鼠的骨髓中尿卟啉积聚。
DOI：
10.1016/0006-2952(85)90299-0
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发表时间：
1985
影响因子：
5.8
通讯作者：
Piper,WN
中科院分区：
文献类型：
--
作者：
Christenson,WR;Reddy,VR;Piper,WN
材料和方法 动物的治疗。将成年雄性 Sprague-Dawley 大鼠 (Sasco, Omaha, NE, 170-200 g) 以三只为一组饲养在吊笼中，并暴露于 12 小时的光/暗周期（6：上午 0 点开灯；下午 6 点：下午 0 点关灯）。随意提供食物（Purina Laboratory Rodent Chow，Ralston-Purina Co.，St. Louis，MO）和水--*为了诱发铅中毒，在第 0 天和第 2 天通过腹腔注射溶解在 0.9% NaCl 中的醋酸铅（30 mg/kg），然后在第 7 天断头。对照接受等体积的 0.9% NaCl (4 ml/kg)。制备用于卟啉分析的组织。快速切除肾脏和肝脏，清洗并在5英寸处用0.9% NaCl灌注。将血液收集在肝素化管中并在5英寸处以2000g离心15分钟。丢弃血浆和白细胞。将红细胞用 0.9% NaCl 在 5" 处悬浮两次，然后在每次悬浮后以 1000 ℃ 离心 10 分钟。用距每只动物股骨 5" 处 0.9% NaCl 冲洗骨髓，并在 5' 处以 1000 8 离心 10 分钟。弃去上清液部分，将每个骨髓沉淀的细胞用0.9% NaCl悬浮两次，然后在每次悬浮后在5'下以1000±8离心10分钟。使用 Potter-Elvehjem 玻璃均质机将每个组织样本（湿重约 1.0 至 1.3 F）在 10 ml 3 N HCl 中均质化-* 所有通信和重印请求均应向其提出。
MATERIALS AND METHODS Treatment of animals. Adult, male Sprague-Dawley rats (Sasco, Omaha, NE, 170-200 g) were housed in hanging cages in groups of three and were exposed to a 12-hr light/dark cycle (6: OO am lights on; 6: OO pm lights off). Food (Purina Laboratory Rodent Chow, Ralston-Purina Co., St. Louis, MO) and water were provided ad lib--* To induce lead poisoning, lead acetate (30 mg/kg), dissolved in 0.9% NaCl, was administered by ip injection at days 0 and 2 followed by decapitation at 7 days. Controls received equivalent volumes of 0.9% NaCl (4 ml/kg).Preparation of tissues for porphyrin analysis. Kidneys and livers were rapidly excised, washed and perfused with 0.9% NaCl at 5". Blood was collected in heparinized tubes and centrifuged at 2000 g for 15 min at 5". The plasma and leukocytes were discarded. Erythrocytes were suspended twice with 0.9% NaCl at 5" followed by centrifugation at 1000 & for 10 min after each suspension. Bone marrow was flushed with 0.9% NaCl at 5" from the femurs of each animal and centrifuged at 1000 8 for 10 min at 5'. The supernatant fraction was discarded and the cells of each marrow pellet were suspended twice with 0.9% NaCl and then centrifuged at 1000 8 for 10 min at 5'after each suspension. Each tissue sample (approximately 1.0 to 1.3 F, wet weight) was homogenized in 10 ml of 3 N HCl using a Potter-Elvehjem glass homog-* To whom all correspondence and reprint requests should be addressed.
铅和遗传因素对人红细胞血红素生物合成的影响*
影响因子：
5.2
作者：
Shigeru Sassa;S. Granick;A. Kappas
通讯作者：
A. Kappas
高效液相色谱法同时测定红细胞中羟甲基胆烷合酶和尿卟啉原Ⅲ合酶。
影响因子：
4.1
作者：
D. Wright;C. Lim
通讯作者：
C. Lim
铅对红细胞和肝尿卟啉原 I 合成酶活性的差异抑制。
影响因子：
6.1
作者：
Walter N. Piper;T. R. Tephly
通讯作者：
T. R. Tephly