Human myeloperoxidase gene: molecular cloning and expression in leukemic cells.
Human myeloperoxidase gene: molecular cloning and expression in leukemic cells.
复制标题
人髓过氧化物酶基因：白血病细胞中的分子克隆和表达。
作者：
K. Chang;J. Trujillo;R. Cook;S. Stass
我们用亲和纯化的髓过氧化物酶(MPO)抗体筛选，从lambda gt11表达文库中克隆了人髓过氧化物酶(MPO)基因。利用MPO基因的cDNA克隆研究了MPO基因在白血病细胞中的表达。从克隆pMP401的核苷酸序列预测的氨基酸序列与60,000个MPO亚基的NH2末端的23个氨基酸序列完全匹配。我们发现，MPO基因与一条19kb的EcoRI基因组条带杂交，表明MPO基因代表人类基因组中的单个基因。对白血病细胞系和急性髓系白血病(AML)患者标本中提取的RNA进行Northern印迹分析表明，MPO基因的表达与髓系有关。Northern印迹显示MPO mRNA表达强度与细胞化学染色MPO表达水平呈正相关。MPO基因的表达种类繁多。这表明，单个MPO基因可能通过转录后加工机制编码不同的RNA物种，或者在MPO基因中存在多个转录起始/终止位点。
We have molecularly cloned the human myeloperoxidase (MPO) gene from the lambda gt11 expression library by screening with an affinity-purified MPO antibody. The cDNA clone of the MPO gene was used to study MPO gene expression in leukemic cells. The amino acid sequence predicted from the nucleotide sequence of the cDNA clone pMP401 matched exactly the 23 amino acid sequence of the NH2-terminal of the 60,000 MPO subunit. We found that MPO cDNA hybridized to a single EcoRI genomic band of 19 kb, indicating that the MPO gene represents a single gene in the human genome. Northern blot analysis of RNA isolated from leukemic cell lines and acute myelogenous leukemia (AML) patients' samples shows that MPO gene expression correlated with myeloid lineage. The intensity of MPO mRNA expression on Northern blot correlated with the level of MPO expression by cytochemical staining. Multiple species of MPO mRNA were found. This indicates that a single MPO gene may encode different RNA species through a mechanism of posttranscriptional processing or that multiple transcriptional start/termination sites exist in the MPO gene.
来自人类中性粒细胞的多种形式的髓过氧化物酶：区室化、酶活性和亚基结构差异的证据。
影响因子：
3.9
作者：
Pember,SO;Shapira,R;KinkadeJr,JM
通讯作者：
KinkadeJr,JM
人中性粒细胞髓过氧化物酶肽亚基的分析。
影响因子：
20.3
作者：
Nauseef,WM;Malech,HL
通讯作者：
Malech,HL